Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Jan 10;24(1):19.
doi: 10.1186/s12866-023-03176-2.

Unique composition of ocular surface microbiome in the old patients with dry eye and diabetes mellitus in a community from Shanghai, China

Zhangling Chen  1   2 , Senlin Lin  3 , Yi Xu  3 , Lina Lu  3 , Haidong Zou  4   5   6   7   8   9
Affiliations

Unique composition of ocular surface microbiome in the old patients with dry eye and diabetes mellitus in a community from Shanghai, China

Zhangling Chen et al. BMC Microbiol. .

Abstract

Background: This study investigates the variations in microbiome abundance and diversity on the ocular surfaces of diabetic patients suffering from dry eye within a community setting. The goal is to offer theoretical insights for the community-level prevention and treatment of dry eye in diabetic cohorts.

Methods: Dry eye screening was performed in the Shanghai Cohort Study of Diabetic Eye Disease (SCODE) from July 15, 2021, to August 15, 2021, in the Xingjing community; this study included both a population with diabetes and a normal population. The population with diabetes included a dry eye group (DM-DE, n = 40) and a non-dry eye group (DM-NoDE, n = 39). The normal population included a dry eye group (NoDM-DE, n = 40) and a control group (control, n = 39). High-throughput sequencing of the 16 S rRNA V3-V4 region was performed on conjunctival swab from both eyes of each subject, and the composition of microbiome on the ocular surface of each group was analyzed.

Results: Significant statistical differences were observed in both α and β diversity of the ocular surface microbiome among the diabetic dry eye, diabetic non-dry eye, non-diabetic dry eye, and normal control groups (P < 0.05).

Conclusions: The study revealed distinct microecological compositions on the ocular surfaces between the diabetic dry eye group and other studied groups. Firmicutes and Anoxybacillus were unique bacterial phyla and genera in the dry eye with DM group, while Actinobacteria and Corynebacterium were unique bacterial phyla and genera in the normal control group.

Keywords: 16S rRNA; Diabetes; Dry eye; Microbiome; Ocular surface.

PubMed Disclaimer

Conflict of interest statement

Competing interests. The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
(A) Sequence length distribution; (B) the number of ASVs/OTUs; (C) ASV/OUT Venn diagram; (D) Rarefaction curve; (E) Species accumulation curves
Fig. 2
Fig. 2
(A) α-Diversity index analysis of the four groups; (B) PCoA analysis; (C) NMDS analysis; (D) the relative abundance of four groups at phyla level (top 10); (E) the relative abundance of four groups at genus level (Top 20); F Species composition heatmap at the genus level (Top 20)
Fig. 3
Fig. 3
(A) LEfSe analysis in four groups; (B) analysis of differences between the four groups; (C) PCA analysis
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Lemp MA, Badouin C, Baum J, et al. The definition and classification of dry eye disease: report of the definition and classification subcommittee of the international dry eye workshop (2007) Ocul Surf. 2007;5(2):75–92. doi: 10.1016/S1542-0124(12)70081-2. - DOI - PubMed
    1. Hom M, De Land P. Self-reported dry eyes and diabetic history. Optometry. 2006;77(11):554–8. doi: 10.1016/j.optm.2006.08.002. - DOI - PubMed
    1. Jeganathan VS, Wang JJ, Wong TY. Ocular associations of diabetes other than diabetic retinopathy. Diabetes Care. 2008;31(9):1905–12. doi: 10.2337/dc08-0342. - DOI - PMC - PubMed
    1. Manaviat MR, Rashidi M, Afkhami-Ardekani M, et al. Prevalence of dry eye syndrome and diabetic retinopathy in type 2 diabetic patients. BMC Ophthalmol. 2008;8:10. doi: 10.1186/1471-2415-8-10. - DOI - PMC - PubMed
    1. Zhang X, Zhao L, Deng S, et al. Eye Syndrome in patients with diabetes Mellitus: prevalence, etiology, and clinical characteristics. J Ophthalmol. 2016;2016:8201053. doi: 10.1155/2016/8201053. - DOI - PMC - PubMed

Publication types