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. 2023 Dec 19;14(1):6.
doi: 10.3390/ani14010006.

Molecular Detection and Genetic Characterization of Vertically Transmitted Viruses in Ducks

Xinrong Wang  1 , Haidong Yu  2 , Wenli Zhang  2 , Lizhi Fu  3 , Yue Wang  1
Affiliations

Molecular Detection and Genetic Characterization of Vertically Transmitted Viruses in Ducks

Xinrong Wang et al. Animals (Basel). .

Abstract

To investigate the distribution and genetic variation in four vertically transmitted duck pathogens, including duck hepatitis B virus (DHBV), duck circovirus (DuCV), duck hepatitis A virus 3 (DHAV-3), and avian reoviruses (ARV), we conducted an epidemiology study using PCR and RT-PCR assays on a duck population. We found that DHBV was the most prevalent virus (69.74%), followed by DuCV (39.48%), and then ARV (19.92%) and DHAV-3 (8.49%). Among the 271 duck samples, two, three or four viruses were detected in the same samples, indicating that the coinfection of vertical transmission agents is common in ducks. The genetic analysis results showed that all four identified DuCV strains belonged to genotype 1, the DHAV-3 strain was closely clustered with previously identified strains from China, and the ARV stain was clustered under genotype 1. These indicate that different viral strains are circulating among the ducks. Our findings will improve the knowledge of the evolution of DuCV, DHAV-3, and ARV, and help choose suitable strains for vaccination.

Keywords: avian reoviruses; duck circovirus; duck hepatitis A virus 3; duck hepatitis B virus; epidemiology.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Part of PCR or RT-PCR results of DuCV (a), DHBV (b), DHAV-3 (c), and ARV (d).
Figure 2
Figure 2
Coinfection rates of DHBV, DuCV, DHAV-3, and ARV.
Figure 3
Figure 3
Sequence analysis of DuCV. (a): Phylogenetic tree of DuCV. Strains detected in this study are marked with “⦁”. (b): Amino acid sequence analysis of DuCV-Cap. Strains detected in this study are marked with red color.
Figure 4
Figure 4
Sequence analysis of DHAV-VP1. (a): Phylogenetic tree of DHAV. (b): Amino acid sequence analysis of DHAV3-VP1. The DHAV strain identified in this study are marked with “⦁”. The strains detected in this study are marked in red.
Figure 5
Figure 5
Sequence analysis of ARV. (a): Phylogenetic tree of ARV. The ARV strain identified in this study are marked with “⦁”. (b) B: Amino acid sequence analysis of ARV-σC. The strain detected in this study is marked in red.
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