Ameliorating Effects of Graphene Oxide on Cadmium Accumulation and Eco-Physiological Characteristics in a Greening Hyperaccumulator (Lonicera japonica Thunb.)

Abstract

Graphene oxide (GO), as a novel carbon-based nanomaterial (CBN), has been widely applied to every respect of social life due to its unique composite properties. The widespread use of GO inevitably promotes its interaction with heavy metal cadmium (Cd), and influences its functional behavior. However, little information is available on the effects of GO on greening hyperaccumulators under co-occurring Cd. In this study, we chose a typical greening hyperaccumulator (Lonicera japonica Thunb.) to show the effect of GO on Cd accumulation, growth, net photosynthesis rate (P_n), carbon sequestration and oxygen release functions of the plant under Cd stress. The different GO-Cd treatments were set up by (0, 10, 50 and 100 mg L^-1) GO and (0, 5 and 25 mg L^-1) Cd in solution culture. The maximum rate of Cd accumulation in the roots and shoots of the plant were increased by 10 mg L^-1 GO (exposed to 5 mg L^-1 Cd), indicating that low-concentration GO (10 mg L^-1) combined with low-concentration Cd (5 mg L^-1) might stimulate the absorption of Cd by L. japonica. Under GO treatments without Cd, the dry weight of root and shoot biomass, P_n value, carbon sequestration per unit leaf area and oxygen release per unit leaf area all increased in various degrees, especially under 10 mg L^-1 GO, were 20.67%, 12.04%, 35% and 28.73% higher than the control. Under GO-Cd treatments, it is observed that the cooperation of low-concentration GO (10 mg L^-1) and low-concentration Cd (5 mg L^-1) could significantly stimulate Cd accumulation, growth, photosynthesis, carbon sequestration and oxygen release functions of the plant. These results indicated that suitable concentrations of GO could significantly alleviate the effects of Cd on L. japonica, which is helpful for expanding the phytoremediation application of greening hyperaccumulators faced with coexistence with environment of nanomaterials and heavy metals.

Keywords: cadmium; graphene oxide; greening plants; hyperaccumulator; response.

Figure 1

The effect of different GO-Cd treatments on Cd contents in root and shoots of L. japonica. The “**” indicates a significant difference compared with the control (p < 0.01), and the “*” indicates a significant difference compared with the control (p < 0.05). Mean ± SD.

Figure 2

The effect of different GO-Cd treatments on root and shoots biomass dry weight (g) of L. japonica. The “**” indicates a significant difference compared with the control (p < 0.01), and the “*” indicates a significant difference compared with the control (p < 0.05). Mean ± SD.

Figure 3

The effect of different GO-Cd treatments on net photosynthesis rate (P_n) in L. japonica. Mean ± SD.