Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Dec 28;13(1):102.
doi: 10.3390/plants13010102.

MtWOX2 and MtWOX9-1 Effects on the Embryogenic Callus Transcriptome in Medicago truncatula

Elizaveta Y Krasnoperova  1 , Varvara E Tvorogova  1   2   3 , Kirill V Smirnov  4 , Elena P Efremova  1 , Elina A Potsenkovskaia  1   2   3 , Anastasia M Artemiuk  1 , Zakhar S Konstantinov  2 , Veronika Y Simonova  2 , Anna V Brynchikova  2 , Daria V Yakovleva  1 , Daria B Pavlova  1 , Ludmila A Lutova  1   2
Affiliations

MtWOX2 and MtWOX9-1 Effects on the Embryogenic Callus Transcriptome in Medicago truncatula

Elizaveta Y Krasnoperova et al. Plants (Basel). .

Abstract

WOX family transcription factors are well-known regulators of plant development, controlling cell proliferation and differentiation in diverse organs and tissues. Several WOX genes have been shown to participate in regeneration processes which take place in plant cell cultures in vitro, but the effects of most of them on tissue culture development have not been discovered yet. In this study, we evaluated the effects of MtWOX2 gene overexpression on the embryogenic callus development and transcriptomic state in Medicago truncatula. According to our results, overexpression of MtWOX2 leads to an increase in callus weight. Furthermore, transcriptomic changes in MtWOX2 overexpressing calli are, to a large extent, opposite to the changes caused by overexpression of MtWOX9-1, a somatic embryogenesis stimulator. These results add new information about the mechanisms of interaction between different WOX genes and can be useful for the search of new regeneration regulators.

Keywords: WOX; callus; somatic embryogenesis; transcription factors.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Effect of transformation with construct for MtWOX2 overexpression on callus growth. (A,B). Boxplots representing the number of somatic embryos per callus (A) or weight (B) of transgenic T0 calli with GUS and MtWOX2 overexpression. Data were obtained from 20–22 calli for different genotypes. To assess the statistical significance of the observed differences, the Wilcoxon signed-rank test was used. (C,D). Boxplots representing the number of somatic embryos per callus (C) or weight (D) of calli obtained from R108 plants and plants containing construct for MtWOX2 overexpression. Data were obtained from 4–7 calli for different genotypes.
Figure 2
Figure 2
Results of principal component analysis of samples taken from GUS and MtWOX2 overexpressing calli. Analysis was performed with the DeSeq2 package.
Figure 3
Figure 3
(A) Venn diagram representing an overlap between DEGs in MtWOX2oe calli in comparison with GUSoe calli and DEGs in MtWOX9-1oe calli in comparison with R108 calli [27]. (B) Overrepresented “Biological process” GO pathways in DEGs upregulated in the MtWOX9-1oe calli and downregulated in the MtWOX2oe calli.
See this image and copyright information in PMC

Similar articles

See all similar articles

References

    1. Loyola-Vargas V.M., Ochoa-Alejo N. An Introduction to Plant Tissue Culture: Advances and Perspectives. In: Loyola-Vargas V.M., Ochoa-Alejo N., editors. Plant Cell Culture Protocols. Springer; New York, NY, USA: 2018. pp. 3–13. Methods in Molecular Biology. - PubMed
    1. Long Y., Yang Y., Pan G., Shen Y. New Insights into Tissue Culture Plant-Regeneration Mechanisms. Front. Plant Sci. 2022;13:926752. doi: 10.3389/fpls.2022.926752. - DOI - PMC - PubMed
    1. Tvorogova V.E., Krasnoperova E.Y., Potsenkovskaia E.A., Kudriashov A.A., Dodueva I.E., Lutova L.A. What Does the WOX Say? Review of Regulators, Targets, Partners. Mol. Biol. 2021;55:311–337. doi: 10.1134/S002689332102031X. - DOI - PubMed
    1. Haecker A., Gross-Hardt R., Geiges B., Sarkar A., Breuninger H., Herrmann M., Laux T. Expression Dynamics of WOX Genes Mark Cell Fate Decisions during Early Embryonic Patterning in Arabidopsis Thaliana. Development. 2004;131:657–668. doi: 10.1242/dev.00963. - DOI - PubMed
    1. Ueda M., Zhang Z., Laux T. Transcriptional Activation of Arabidopsis Axis Patterning Genes WOX8/9 Links Zygote Polarity to Embryo Development. Dev. Cell. 2011;20:264–270. doi: 10.1016/j.devcel.2011.01.009. - DOI - PubMed

LinkOut - more resources