Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024;19(1):39-45.
doi: 10.22037/iej.v19i1.42608.

Niosomal Doxycycline and Triamcinolone: A Novel Approach to Minimize Cytotoxicity in Endodontic Medicaments

Rahim Fereidooni  1 , Arash Shahravan  1 , Hamed Manochehrifar  1 , Abbas Pardakhty  2 , Fatemeh Nosrati  3 , Hamid Reza Mollaie  4
Affiliations

Niosomal Doxycycline and Triamcinolone: A Novel Approach to Minimize Cytotoxicity in Endodontic Medicaments

Rahim Fereidooni et al. Iran Endod J. 2024.

Abstract

Introduction: Mechanical root canal preparations and irrigation solutions are essential for reducing microbial counts in the root canal system. However, these methods do not completely eliminate microorganisms. Intracanal medicaments are used to further decrease microbial counts. This study aims to assess the cytotoxicity of various intracanal medicaments.

Materials and methods: In this in vitro study, murine fibroblast cell lines (L929) were cultured in a controlled environment. The MTT assay was employed to evaluate the cytotoxicity of different medicament combinations, including calcium hydroxide and triamcinolone (D1), niosomal doxycycline and triamcinolone (D2), calcium hydroxide (D3), and a combination of doxycycline and triamcinolone (D4). Statistical analysis was performed using ANOVA and Dunnett's test.

Results: The results indicated that D1 and D2 had lower cytotoxicity, while D4 exhibited the highest cytotoxicity. D1 was found to be non-cytotoxic up to a concentration of 500 µg/mL over a period of 72 hours. D2 and D3 showed similar effects up to concentrations of 250 µg/mL and 100 µg/mL, respectively, for 72 hours. In contrast, D4 exhibited cytotoxicity at concentrations above 75 µg/mL at 72 hours.

Conclusion: This study suggests that encapsulating doxycycline in niosomal structures (D2) reduces cytotoxicity in murine fibroblast cell lines (L929) for at least 24 and 48 hours. These findings offer promising implications for the development of endodontic medicaments with improved biocompatibility.

Keywords: Calcium Hydroxide; Corticosteroid; Cytotoxicity; Niosomal Doxycycline.

PubMed Disclaimer

Conflict of interest statement

None.

Figures

Figure 1
Figure 1
A, C) The cytotoxicity of different concentrations; B, D) Comparison of medications at maximum nontoxic concentration with the control group
Figure 2
Figure 2
A, C) The cytotoxicity of different concentrations; B, D) Comparison of medications at maximum nontoxic concentration with the control group
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Hübscher W, Barbakow F, Peters OA. Root‐canal Preparation with Flexmaster: Canal Shapes Analysed by Micro‐Computed Tomography. Int Endod J. 2003;36(11):740–7. - PubMed
    1. Kennedy J, Hussey D. The Antimicrobial Effects of Root Canal Irrigation and Medication. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2007;103(4):560–9. - PubMed
    1. Awawdeh L, AL‐Beitawi M, Hammad M. Effectiveness of Propolis and Calcium Hydroxide as a Short‐Term Intracanal Medicament Against Enterococcus Faecalis: A Laboratory Study. Aust Endod J. 2009;35(2):52–8. - PubMed
    1. Peters OA, Peters CI, Schönenberger K, Barbakow F. ProTaper Rotary Root Canal Preparation: Assessment of Torque and Force in Relation to Canal Anatomy. Int Endod J. 2003;36(2):93–9. - PubMed
    1. Ramos IF, Biz MT, Paulino N, Scremin A, Della Bona A, Barletta FB, Figueiredo JA. Histopathological Analysis of Corticosteroid-Antibiotic Preparation and Propolis Paste Formulation as Intracanal Medication after Pulpectomy: An in vivo Study. J Appl Oral Sci. 2012;20(1):50–6. - PMC - PubMed

LinkOut - more resources