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. 2023 Aug 31;78(4):1349-1357.
doi: 10.32592/ARI.2023.78.4.1349. eCollection 2023 Aug.

Molecular Investigation of Brucella Species Belongs to Sheep and Goats in Seropositive Samples from an Endemic Area of Hamedan Province; Famenin Brucellosis Cohort Study

M Adabi  1 , S Alamian  2 , M Varasteh-Shams  1 , H Ghaderi  3 , F Shahbazi  4 , J Gharekhani  5
Affiliations

Molecular Investigation of Brucella Species Belongs to Sheep and Goats in Seropositive Samples from an Endemic Area of Hamedan Province; Famenin Brucellosis Cohort Study

M Adabi et al. Arch Razi Inst. .

Abstract

Brucellosis is a zoonotic infectious disease in western regions of Iran, especially in Hamedan province. Following the Famenin brucellosis cohort study, the main aim of the current study was the molecular detection of Brucella species (spp.) in sheep and goats from Famenin, Hamedan, Iran. A total of 23 Brucella-seropositive samples (sheep=21 and goats=2), which had been screened from 1,660 animals in the Famenin cohort study, were used to detect Brucella-DNA using the BCSP31 target gene and IS711 locus. In total, 20 of 23 samples were positive for Brucella infection by using specific primers. Additionally, Brucella melitensis (B. melitensis) and Brucella abortus (B. abortus) were confirmed in 90% (n=18) and 10% (n=2) of positive samples, respectively. There was no sample with the co-infection of B. abortus and B. melitensis. In this study, B. abortus was isolated from one of the goat samples. This is the first report on Brucella spp. in animals in the region. It was found that B. melitensis is the dominant spp. responsible for brucellosis in animals from Famenin. Molecular techniques are reliable tools to detect Brucella infection, especially in cases without serology findings and conclusive results.

Keywords: Animal; Famenin; Molecular detection; Brucella abortus; Brucella melitensis.

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Conflict of interest statement

The authors declare that they have no conflict of interest.

Figures

Figure 1
Figure 1
Geographic location of Hamedan province and Famenin, studied area
Figure 2
Figure 2
The PCR products electrophoresis to detect of Brucella genus. Lane M: 100 bp marker; lane C+: B. melitensis standard strain (Razi vaccine and serum research institute) as positive control; lane C-: physiological serum as negative control. Lanes 1 to 12: positive samples
Figure 3
Figure 3
The PCR products electrophoresis to detect of Brucella abortus. Lane M: 100 bp marker; lane C+: B. abortus standard strain (Razi vaccine and serum research institute) as positive control; lane C-: physiological serum as negative control. Lanes 1 to 5: positive samples
Figure 4
Figure 4
The PCR products electrophoresis to detect of Brucella melitensis. Lane M: 100 bp marker; lane C+: B. melitensis standard strain (Razi vaccine and serum research institute) as positive control; lane C-: physiological serum as negative control. Lanes 1 to 14: positive samples
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