Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1987 Jan 26;15(2):397-416.
doi: 10.1093/nar/15.2.397.

The final deprotection step in oligonucleotide synthesis is reduced to a mild and rapid ammonia treatment by using labile base-protecting groups

J C Schulhof, D Molko, R Teoule
Free PMC article
Comparative Study

The final deprotection step in oligonucleotide synthesis is reduced to a mild and rapid ammonia treatment by using labile base-protecting groups

J C Schulhof et al. Nucleic Acids Res. .
Free PMC article

Abstract

Phenoxyacetyl (pac) and methoxyacetyl (mac) for adenine and guanine, isobutyryl for cytosine, were successfully applied as amino protecting groups both in phosphotriester and phosphoramidite approaches. As shown by N.M.R. and H.P.L.C. analysis, they are completely deblocked in less than four hours in 29% ammonia at room temperature allowing the preparation of modified DNA containing alkali labile bases such as saturated pyrimidines. The stability of N6-phenoxyacetyl-deoxyadenosine versus depurination in acidic conditions used in the detritylation step was favorably compared with that of the classic N6-benzoyl protected adenine.

PubMed Disclaimer

References

    1. J Mol Biol. 1972 Dec 28;72(2):251-88 - PubMed
    1. Methods Enzymol. 1980;65(1):499-560 - PubMed
    1. Nucleic Acids Symp Ser. 1980;(7):281-91 - PubMed
    1. Nucleic Acids Res. 1982 Nov 11;10(21):6675-94 - PubMed
    1. Anal Biochem. 1983 Feb 1;128(2):281-6 - PubMed

Publication types

MeSH terms