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. 1987 Jan 26;15(2):797-811.
doi: 10.1093/nar/15.2.797.

Dissociation kinetics of 19 base paired oligonucleotide-DNA duplexes containing different single mismatched base pairs

S Ikuta, K Takagi, R B Wallace, K Itakura
Free PMC article

Dissociation kinetics of 19 base paired oligonucleotide-DNA duplexes containing different single mismatched base pairs

S Ikuta et al. Nucleic Acids Res. .
Free PMC article

Abstract

The dissociation kinetics of 19 base paired oligonucleotide-DNA duplex containing a various single mismatched base pair are studied on dried agarose gels. The kinetics of the dissociation are first order under our experimental conditions. The incorporation of a single mismatched base pair destabilizes the DNA duplexes to some extent, the amount depending on the nature of the mismatched base pair. G-T and G-A mismatches slightly destabilize a duplex, while A-A, T-T, C-T and C-A mismatches significantly destabilize it. The activation energy for the overall dissociation processes for these oligonucleotide-DNA duplexes containing 19 base pairs is 52 +/- 2 Kcal mol-1 as determined from the slope of Arrhenius plot.

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