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. 2023 Nov 30;12(23):4329.
doi: 10.3390/foods12234329.

Snapshot of the Probiotic Potential of Kluveromyces marxianus DMKU-1042 Using a Comparative Probiogenomics Approach

Mati Ullah  1 , Muhammad Rizwan  2 , Ali Raza  3 , Yutong Xia  1 , Jianda Han  1 , Yi Ma  1 , Huayou Chen  1
Affiliations

Snapshot of the Probiotic Potential of Kluveromyces marxianus DMKU-1042 Using a Comparative Probiogenomics Approach

Mati Ullah et al. Foods. .

Abstract

Kluyveromyces marxianus is a rapidly growing thermotolerant yeast that secretes a variety of lytic enzymes, utilizes different sugars, and produces ethanol. The probiotic potential of this yeast has not been well explored. To evaluate its probiotic potential, the yeast strain Kluyveromyces marxianus DMKU3-1042 was analyzed using next-generation sequencing technology. Analysis of the genomes showed that the yeast isolates had a GC content of 40.10-40.59%. The isolates had many genes related to glycerol and mannose metabolism, as well as genes for acetoin and butanediol metabolism, acetolactate synthase subunits, and lactic acid fermentation. The strain isolates were also found to possess genes for the synthesis of different vitamins and Coenzyme A. Genes related to heat and hyperosmotic shock tolerance, as well as protection against reactive oxygen species were also found. Additionally, the isolates contained genes for the synthesis of lysine, threonine, methionine, and cysteine, as well as genes with anticoagulation and anti-inflammatory properties. Based on our analysis, we concluded that the strain DMKU3-1042 possesses probiotic properties that make it suitable for use in food and feed supplementation.

Keywords: Kluyveromyces marxianus; fermentation; genes; next-generation sequencing; probiotic; yeast.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Whole-genome-based phylogenetic tree of 5 isolates of Kluyveromyces marxianus DMKU3-1042 genomes compared to the genomes of other strains. To generate the support values of the tree, 100 rounds of RAxML Fast Bootstrapping option were used.
Figure 2
Figure 2
Whole-genome comparison of Kluyveromyces marxianus DMKU3-1042 genomes with other yeast strains.
Figure 3
Figure 3
Functional categorization of all predicted coding sequences (CDS) in Kluyveromyces marxianus strains.
Figure 4
Figure 4
Relative abundance of sugars between K. marxianus strain DMKU3-1042 isolates (S1, S2, S3, S4, S5) and other investigated strains in this study.
Figure 5
Figure 5
Relative abundance of vitamins, coenzymes, and prosthetic groups across K. marxianus strains in this study.
Figure 6
Figure 6
The percentage frequency of the three forms of glutathione across K. marxianus strains.
See this image and copyright information in PMC

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