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. 2024 Mar;204(3):e28-e30.
doi: 10.1111/bjh.19300. Epub 2024 Jan 17.

Sickle red blood cells directly activate neutrophils

Grace M Lee  1 , Milena Batchvarova  1 , Martha Delahunty  1 , Lydia Boateng  2 , Kimberly Boyle  1 , Mark A Suggs  1 , Marilyn J Telen  1   3   4
Affiliations

Sickle red blood cells directly activate neutrophils

Grace M Lee et al. Br J Haematol. 2024 Mar.
No abstract available

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Conflict of interest statement

The authors declare no competing financial interests.

Figures

Figure 1.
Figure 1.. Evidence of neutrophil activation in normal blood donors and blood of patients with SCD.
A. Adherence of neutrophils to HUVECs from healthy (n=19) or SCD subjects (n=7) under flow at 1 dyne/cm2. B/C. MMP9 release after whole blood from healthy or SCD subjects was incubated with buffer (Panel B) or fMLF (Panel C, 1 μM, n=19) for 30 minutes. ***p<0.001, **** p<0.0001
Figure 2.
Figure 2.. Intact SSRBCs but not AARBCs stimulate neutrophil adhesion and degranulation.
A. Neutrophils from healthy donors were incubated with AA normal (n=3) or SSRBCs (n=22) after treatment with sham or epinephrine (20 μM) for 30 minutes. After red cells were lysed and remaining cells were washed, neutrophils were infused into the flow chamber and adherence to HUVECs was quantified. B. Normal neutrophils were incubated with buffer or SSRBCs (n=7) for 30 minutes, and MMP9 release was measured. *p<0.05, **p<0.01, ***p<0.001, **** p<0.0001
See this image and copyright information in PMC

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