. 2024 Mar;43(3):551-557.

doi: 10.1007/s10096-024-04752-8. Epub 2024 Jan 18.

Molecular analysis of metallo-beta-lactamase-producing Pseudomonas aeruginosa in Switzerland 2022-2023

Jacqueline Findlay¹, Otavio Hallal Ferreira Raro², Laurent Poirel^{2

3}, Patrice Nordmann^{2

3}; NARA Network

Collaborators, Affiliations

Collaborators

NARA Network:
R Lienhard, L Vonallmen, C Schilt, A Scherler, K Lucke, M Jutzi, M Reichmuth, U Schibli, C Fricker, S Pranghofer, G Greub, D Blanc, A Vitale, B Lemaire, M Fatoux, M Tritten, L Rumebe, N Liassine, G Jost, N Wohlwend, D Schultze, K Burren, A Westers, M Imperiali, L Pozzi, D Balzari, G Vaninetti, C Cirillo, V Gaia, E Pianezzi, G Martinetti Lucchini, F Baggi Menozzi, A Jayol, C Guyon, D Hyden, M Maitrejean, V Deggi-Messmer, D Bandeira, C Fournier, S Pfister, C Nusbaumer, L Bertaiola Monnerat, J Schrenzel, G Renzi, A Cherkaoui, D Andrey, S Emonet, M Eyer, R Maret, A Belo, D Mabillard, M Moraz, K Herzog, V Gisler, E Hitz, M Oberle, C Castelberg, H Fankhauser, S Graf, N Dubey, C Guler, M Schoenenberger, U Karrer, F Piran, C Andreutti, M Dessauges, T Schmid, B Suterbuser, I Mitrovic, E Gruner, V Bruderer, P Staehli, B Schnell, C O Marti, I Steffen, A Imhof, B Preiswerk, V Dilorenzo, C Payen, D Boschung, L Comte, M Schacher, M Brandenberger, C Zowa, C Zehnder, B Mathis, L Basilico, G Togni, P Minkova, Y Born, M Kuegler, V Povolo, S Droz, M Elzi, C Casanova, D Goldenberger, P Keller, C Lang, A Blaich, S Schmid, B Ivan, A Egli, S Mancini, O Dubois, K Narr, S Schoch, S Ellenberger, S Seiffert

Affiliations

¹ Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Chemin du Musée 18, CH-1700, Fribourg, Switzerland. Jacqueline.findlay@unifr.ch.
² Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Chemin du Musée 18, CH-1700, Fribourg, Switzerland.
³ Swiss National Reference Center for Emerging Antibiotic Resistance (NARA) Network, Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Fribourg, Switzerland.

PMID: 38233610
PMCID: PMC10917820
DOI: 10.1007/s10096-024-04752-8

Molecular analysis of metallo-beta-lactamase-producing Pseudomonas aeruginosa in Switzerland 2022-2023

Jacqueline Findlay et al. Eur J Clin Microbiol Infect Dis. 2024 Mar.

. 2024 Mar;43(3):551-557.

doi: 10.1007/s10096-024-04752-8. Epub 2024 Jan 18.

Authors

Jacqueline Findlay¹, Otavio Hallal Ferreira Raro², Laurent Poirel^{2

3}, Patrice Nordmann^{2

3}; NARA Network

Collaborators

NARA Network:
R Lienhard, L Vonallmen, C Schilt, A Scherler, K Lucke, M Jutzi, M Reichmuth, U Schibli, C Fricker, S Pranghofer, G Greub, D Blanc, A Vitale, B Lemaire, M Fatoux, M Tritten, L Rumebe, N Liassine, G Jost, N Wohlwend, D Schultze, K Burren, A Westers, M Imperiali, L Pozzi, D Balzari, G Vaninetti, C Cirillo, V Gaia, E Pianezzi, G Martinetti Lucchini, F Baggi Menozzi, A Jayol, C Guyon, D Hyden, M Maitrejean, V Deggi-Messmer, D Bandeira, C Fournier, S Pfister, C Nusbaumer, L Bertaiola Monnerat, J Schrenzel, G Renzi, A Cherkaoui, D Andrey, S Emonet, M Eyer, R Maret, A Belo, D Mabillard, M Moraz, K Herzog, V Gisler, E Hitz, M Oberle, C Castelberg, H Fankhauser, S Graf, N Dubey, C Guler, M Schoenenberger, U Karrer, F Piran, C Andreutti, M Dessauges, T Schmid, B Suterbuser, I Mitrovic, E Gruner, V Bruderer, P Staehli, B Schnell, C O Marti, I Steffen, A Imhof, B Preiswerk, V Dilorenzo, C Payen, D Boschung, L Comte, M Schacher, M Brandenberger, C Zowa, C Zehnder, B Mathis, L Basilico, G Togni, P Minkova, Y Born, M Kuegler, V Povolo, S Droz, M Elzi, C Casanova, D Goldenberger, P Keller, C Lang, A Blaich, S Schmid, B Ivan, A Egli, S Mancini, O Dubois, K Narr, S Schoch, S Ellenberger, S Seiffert

Affiliations

¹ Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Chemin du Musée 18, CH-1700, Fribourg, Switzerland. Jacqueline.findlay@unifr.ch.
² Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg, Chemin du Musée 18, CH-1700, Fribourg, Switzerland.
³ Swiss National Reference Center for Emerging Antibiotic Resistance (NARA) Network, Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg, Fribourg, Switzerland.

PMID: 38233610
PMCID: PMC10917820
DOI: 10.1007/s10096-024-04752-8

Abstract

Objectives: The occurrence of metallo-beta-lactamase-producing Pseudomonas aeruginosa (MBL-PA) isolates is increasing globally, including in Switzerland. The aim of this study was to characterise, phenotypically and genotypically, the MBL-PA isolates submitted to the Swiss National Reference Center for Emerging Antibiotic Resistance (NARA) reference laboratory over a 12-month period from July 2022 to July 2023.

Methods: Thirty-nine non-duplicate MBL-PA Isolates were submitted to NARA over the study period from across Switzerland. Susceptibility was determined by broth microdilution according to EUCAST methodology. Whole-genome sequencing was performed on 34 isolates. Sequence types (STs) and resistance genes were ascertained using the Centre for Genomic Epidemiology platform. MBL genes, bla_NDM-1, bla_IMP-1, and bla_VIM-2, were cloned into vector pUCP24 and transformed into P. aeruginosa PA14.

Results: The most prevalent MBL types identified in this study were VIM (21/39; 53.8%) followed by NDM (11/39; 28.2%), IMP (6/39; 15.4%), and a single isolate produced both VIM and NDM enzymes. WGS identified 13 different STs types among the 39 isolates. They all exhibited resistance to cephalosporins, carbapenems, and the beta-lactam-beta-lactamase inhibitor combinations, ceftolozane-tazobactam, ceftazidime-avibactam, imipenem-relebactam, and meropenem-vaborbactam, and 8 isolates were cefiderocol (FDC) resistant. Recombinant P. aeruginosa strains producing bla_NDM-1, bla_IMP-1, and bla_VIM-2 exhibited FDC MICs of 16, 8, and 1 mg/L, respectively.

Conclusions: This study showed that the MBL-PA in Switzerland could be attributed to the wide dissemination of high-risk clones that accounted for most isolates in this study. Although FDC resistance was only found in 8 isolates, MBL carriage was shown to be a major contributor to this phenotype.

Keywords: Pseudomonas aeruginosa; Carbapenems; Epidemiology; Metallo-beta-lactamase.

PubMed Disclaimer

Conflict of interest statement

The authors declare no competing interests.

Figures

**Fig. 1**
Core genome alignment of 34 MBL-producing *P. aeruginosa* strains with STs, MBL variants, Canton of origin and OprF status. NF; non-functional, blank; functional

See this image and copyright information in PMC

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Molecular analysis of metallo-beta-lactamase-producing Pseudomonas aeruginosa in Switzerland 2022-2023

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Molecular analysis of metallo-beta-lactamase-producing Pseudomonas aeruginosa in Switzerland 2022-2023

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