Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Jan 18;191(2):104.
doi: 10.1007/s00604-023-06126-x.

A nanogap-enhanced SERS nanotag-based lateral flow assay for ultrasensitive and simultaneous monitoring of SARS-CoV-2 S and NP antigens

Xiaoxian Liu #  1   2 , Xingsheng Yang #  1   3 , Chongwen Wang  4 , Qiqi Liu  3 , Yanlei Ding  3 , Shiping Xu  5 , Guanghui Wang  6 , Rui Xiao  7
Affiliations

A nanogap-enhanced SERS nanotag-based lateral flow assay for ultrasensitive and simultaneous monitoring of SARS-CoV-2 S and NP antigens

Xiaoxian Liu et al. Mikrochim Acta. .

Abstract

A lateral flow assay (LFA) strip based on dual 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB)-encoded satellite Fe3O4@Au (Mag@Au) SERS tags with nanogap is reported for ultrasensitive and simultaneous diagnosis of two SARS-CoV-2 functional proteins. Composed of Fe3O4 core, satellite gold shell with nanogaps, and double-layer DTNB, the Mag@Au nanoparticles with an average size of 238 nm were designed as multifunctional tags to efficiently enrich the target SARS-CoV-2 protein from complex samples, significantly enhancing the SERS signal of the LFA strip and provide quantitative SERS detection of analyte on test lines. The developed dual DTNB-encoded satellite Mag@Au-based LFA allowed simultaneous quantification of spike (S) protein and nucleocapsid (NP) protein with detection limits of 23 pg mL-1 and 2 pg mL-1, respectively, lower than commercial ELISA kits and reported SERS-LFA detection system-based Au NPs and Fe3O4@3 nm Au MNPs. This magnetic SERS-LFA also showed high performance of multi-variant strain detection and further distinguished clinical samples of Omicron variant infection, demonstrating the potential of in situ detection of respiratory virus diseases.

Keywords: Antigen testing; Functional proteins; Lateral flow assay; Multi-variant strain detection; SARS-CoV-2; Satellite Mag@Au SERS tags.

PubMed Disclaimer

References

    1. Lavine JS, Bjornstad ON, Antia R (2021) Immunological characteristics govern the transition of COVID-19 to endemicity. Science 371:741–745. https://doi.org/10.1126/science.abe6522 - DOI - PubMed - PMC
    1. Carabelli AM, Peacock TP, Thorne LG et al (2023) SARS-CoV-2 variant biology: immune escape, transmission and fitness. Nat Rev Microbiol 21:162–177. https://doi.org/10.1038/s41579-022-00841-7 - DOI - PubMed - PMC
    1. Alizadehsani R, Alizadeh Sani Z, Behjati M et al (2021) Risk factors prediction, clinical outcomes, and mortality in COVID-19 patients. J Med Virol 93:2307–2320. https://doi.org/10.1002/jmv.26699 - DOI - PubMed
    1. Wang C, Yang X, Zheng S, Cheng X, Xiao R, Li Q, Wang W, Liu X, Wang S (2021) Development of an ultrasensitive fluorescent immunochromatographic assay based on multilayer quantum dot nanobead for simultaneous detection of SARS-CoV-2 antigen and influenza A virus. Sens Actuators B Chem 345:130372. https://doi.org/10.1016/j.snb.2021.130372 - DOI - PubMed - PMC
    1. Ganesh B, Rajakumar T, Malathi M, Manikandan N, Nagaraj J, Santhakumar A, Elangovan A, Malik YS (2021) Epidemiology and pathobiology of SARS-CoV-2 (COVID-19) in comparison with SARS, MERS: an updated overview of current knowledge and future perspectives. Clin Epidemiol Glob Health 10:100694. https://doi.org/10.1016/j.cegh.2020.100694 - DOI - PubMed - PMC

Publication types

Supplementary concepts