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Comment
. 2024 Mar;102(3):153-155.
doi: 10.1111/imcb.12724. Epub 2024 Jan 18.

A new way of identifying viral pathogens reactivating in cellular therapy products

Anthony L Cunningham  1 , Kenneth Micklethwaite  1
Affiliations
Comment

A new way of identifying viral pathogens reactivating in cellular therapy products

Anthony L Cunningham et al. Immunol Cell Biol. 2024 Mar.

Abstract

In this article, we discuss a recently published article that demonstrated a novel way of identifying viral pathogens reactivating in human cells to be used as cellular therapy, in this instance chimeric antigen receptor (CAR) T cells. The authors used search engines and databases to identify viruses able to reactivate in T cells and then tested this initially in T-cell cultures, specifically human herpesvirus 6. This virus was then shown to reactivate infrequently in vitro and in vivo in CAR T cells as a consequence of T-cell activation. The methodology may be most clinically useful for more frequently reactivating viruses in other types of cellular therapy such as allogenic CAR T cells or induced pluripotent stem cells.

Keywords: CAR T cells; HHV-6; cellular therapy; screening; virus reactivation.

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  • Latent human herpesvirus 6 is reactivated in CAR T cells.
    Lareau CA, Yin Y, Maurer K, Sandor KD, Daniel B, Yagnik G, Peña J, Crawford JC, Spanjaart AM, Gutierrez JC, Haradhvala NJ, Riberdy JM, Abay T, Stickels RR, Verboon JM, Liu V, Buquicchio FA, Wang F, Southard J, Song R, Li W, Shrestha A, Parida L, Getz G, Maus MV, Li S, Moore A, Roberts ZJ, Ludwig LS, Talleur AC, Thomas PG, Dehghani H, Pertel T, Kundaje A, Gottschalk S, Roth TL, Kersten MJ, Wu CJ, Majzner RG, Satpathy AT. Lareau CA, et al. Nature. 2023 Nov;623(7987):608-615. doi: 10.1038/s41586-023-06704-2. Epub 2023 Nov 8. Nature. 2023. PMID: 37938768 Free PMC article.

References

REFERENCES

    1. Lareau CA, Yin Y, Maurer K, et al. Latent human herpesvirus 6 is reactivated in CAR T cells. Nature 2023; 623: 608-615.
    1. Spanjaart AM, van der Valk FM, van Rooijen G, Brouwer MC, Kersten MJ. Confused about confusion. N Engl J Med 2022; 386: 80-87.
    1. Locke FL, Ghobadi A, Jacobson CA, et al. Long-term safety and activity of axicabtagene ciloleucel in refractory large B-cell lymphoma (ZUMA-1): a single-arm, multicentre, phase 1-2 trial. Lancet Oncol 2019; 20: 31-42.
    1. Baird JH, Epstein DJ, Tamaresis JS, et al. Immune reconstitution and infectious complications following axicabtagene ciloleucel therapy for large B-cell lymphoma. Blood Adv 2021; 5: 143-155.
    1. Maude SL, Laetsch TW, Buechner J, et al. Tisagenlecleucel in children and young adults with B-cell lymphoblastic leukemia. N Engl J Med 2018; 378: 439-448.

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