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. 2024 Jan;14(1):e1555.
doi: 10.1002/ctm2.1555.

Snai2-mediated upregulation of NADSYN1 promotes bladder cancer progression by interacting with PHB

Li-Juan Jiang  1   2 , Song-Bin Guo  1   3 , Zhao-Hui Zhou  1   2 , Zhi-Yong Li  1   2 , Fang-Jian Zhou  1   2 , Chun-Ping Yu  1   2 , Mei Li  1   4 , Wei-Juan Huang #  5   6 , Zhuo-Wei Liu #  1   2 , Xiao-Peng Tian #  1   3
Affiliations

Snai2-mediated upregulation of NADSYN1 promotes bladder cancer progression by interacting with PHB

Li-Juan Jiang et al. Clin Transl Med. 2024 Jan.
No abstract available

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Conflict of interest statement

The authors declare no competing financial interesting.

Figures

FIGURE 1
FIGURE 1
Delineation of RNA binding by PHB in paired bladder cancer tissues and adjacent normal tissues by RNA immunoprecipitation sequencing (RIP‐SEQ) assays. (A) The Venn diagram illustrates the variation in gene numbers between MIBC and NMIBC. By intersecting three different groups, we pinpointed 10 specific genes that exhibited differential expression. (B) Volcano map of PHB‐associated RNAs in tumour tissues versus normal tissues. (C) Motif analysis of enriched peaks in tumour sample. Peak distribution of genes in the gene body in tumour sample 1 (T1) and adjacent normal tissue sample 1 (N1), tumour sample 2 (T2) and adjacent normal tissue sample 2 (N2). (D) Representative agarose gel electrophoresis images of NADSYN1 (upper panel), LINC01410 (mid panel) and MIR339 (lower panel) in tumour and normal samples identified by RIP‐SEQ followed by PCR. MIBC, muscle‐invasive bladder cancer; NMIBC, non‐muscle‐invasive bladder cancer; FDR, false discovery rate. T, tumour tissue; N, adjacent normal tissue; PHB, Prohibitin. T3, tumour sample 3; N3, adjacent normal tissue sample 3.
FIGURE 2
FIGURE 2
PHB domain is responsible for PHB‐NADSYN1 interaction. (A) Schematic diagram of PHB truncations. (B) Analysis of the binding of different isoforms of NADSYN1 by PHB. (C) and (D) Colony formation assay and trans‐well invasion assay were carried with cell lines. PHB‐△CC, PHB protein lacking CC domain. PHB‐△HTCC, PHB protein lacking HT domain and CC domain. PHB‐△PHB, PHB protein lacking PHB domain. * P < .05, ** P < .01, *** P < .001.
FIGURE 3
FIGURE 3
NADSYN1 promotes PHB stability in bladder cancer. (A) RNA pull‐down assay was carried with different combinations of RNAs indicated. (B) Western blotting assays were carried out in cell lines indicated. (C) Western blot analysis of ubiquitin in different cell lines indicated. The protein stability of PHB was valued in cells with different CHX treatment span. (D) Western blot analysis of ubiquitin in different cell lines expressing different mutants of PHB. PHB stability in cell lines expressing PHB K202A mutant.
See this image and copyright information in PMC

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