Investigation of elemental composition in red teff grains using inductively coupled plasma optical emission spectroscopy (ICP OES), Sire District, Arsi zone, Ethiopia

Abstract

Background: Minerals are important not only for better plant growth and development but also for human and animal nutrition. It is known that east and west Gojam in the Amhara region and east and west Shoa areas in the Oromia region Ethiopia's most teff growing areas. However, there is no information on the mineral content and nutritional worth of Teff Sire district, Arsi zone, Ethiopia. Since ICP OES is a powerful technique to examine elemental compositions even in lower concentration, it is used in this work to investigate the elemental composition of red teff samples.

Methods: The elemental compositions of red Teff grain samples were determined using ICP-OES from three sites: S1, S2, and S3 of Sire district, Arsi zone, Ethiopia. Wet digestion of the teff samples was carried out by weighing 0.5 g red teff sample and digested with 8 ml HNO₃ and 2 ml H₂O₂ (30%) for 3:00 h at a temperature of 100℃ on hot plate. The investigations of method validation, limit of detection and limit of quantification were also carried out.

Results: The average amount of elements in red teff sample obtained as 172-280 mg/kg Fe, 13-76 mg/kg Mn, 8.2-8.5 mg/kg Cu, 24-26 mg/kg Zn, and toxic trace elements 0.12-0.29 mg/kg Pb and 0.15-0.22 mg/kg Cd. The limit of detection found in ranges from 0.21 mg Kg^-1 to 10.44 mg Kg^-1 whereas quantification limit resulted in 0.7 mg Kg^-1 to 34.8 mg Kg^-1 for the metals under consideration. The method was validated by its linear range in the concentration range of 0.028-1.4 ppm or 0.056-2.8 ppm and excellent recovery result was achieved in the range of 90-120%.

Conclusion: This study aimed to investigate the mineral content in red teff cultivated in Ethiopia specifically Arsi zone by using ICP OES. From the obtained results, Iron was the first abundant essential element in red teff compared to Mn, Cu and Zn. The level of trace elements: Cd and Pb in the samples slightly above the acceptable limit, possibly due to agricultural practices like usage of fertilizers, pesticides, and other industrial products. Overall, this red teff elemental composition information contributes to the nutrition database and food safety in Ethiopia and beyond.

Keywords: Elemental composition; Ethiopia; ICP OES; Iron; Red teff.