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. 2024 Jan 2;14(1):79-95.
doi: 10.3390/jox14010005.

Effect of Plant Growth-Promoting Bacteria on Antioxidant Status, Acetolactate Synthase Activity, and Growth of Common Wheat and Canola Exposed to Metsulfuron-Methyl

Margarita Bakaeva  1 , Sergey Chetverikov  1 , Sergey Starikov  1 , Aliya Kendjieva  1 , Gaisar Khudaygulov  1 , Darya Chetverikova  1
Affiliations

Effect of Plant Growth-Promoting Bacteria on Antioxidant Status, Acetolactate Synthase Activity, and Growth of Common Wheat and Canola Exposed to Metsulfuron-Methyl

Margarita Bakaeva et al. J Xenobiot. .

Abstract

Metsulfuron-methyl, a widely used herbicide, could cause damage to the sensitive plants in crop-rotation systems at extremely low levels in the soil. The potential of plant growth-promoting bacteria (PGPB) for enhancing the resistance of plants against herbicide stress has been discovered recently. Therefore, it is poorly understood how physiological processes occur in plants, while PGPB reduce the phytotoxicity of herbicides for agricultural crops. In greenhouse studies, the effect of strains Pseudomonas protegens DA1.2 and Pseudomonas chlororaphis 4CH on oxidative damage, acetolactate synthase (ALS), enzymatic and non-enzymatic antioxidants in canola (Brassica napus L.), and wheat (Triticum aestivum L.) were investigated under two levels (0.05 and 0.25 mg∙kg-1) of metsulfuron-methyl using spectrophotometric assays. The inoculation of herbicide-exposed wheat with bacteria significantly increased the shoots fresh weight (24-28%), amount of glutathione GSH (60-73%), and flavonoids (5-14%), as well as activity of ascorbate peroxidase (129-140%), superoxide dismutase SOD (35-49%), and ALS (50-57%). Bacterial treatment stimulated the activity of SOD (37-94%), ALS (65-73%), glutathione reductase (19-20%), and the accumulation of GSH (61-261%), flavonoids (17-22%), and shoots weight (27-33%) in herbicide-exposed canola. Simultaneous inoculation prevented lipid peroxidation induced by metsulfuron-methyl in sensitive plants. Based on the findings, it is possible that the protective role of bacterial strains against metsulfuron-metil is linked to antioxidant system activation.

Keywords: acetolactate synthase; antioxidant enzymes; antioxidants; bacteria; biostimulant; herbicide; metsulfuron-methyl; safeners.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
The scheme of wheat (A) and rapeseed (B) treatments. The figure does not indicate the location of the pots in the greenhouse.
Figure 2
Figure 2
Effect of strains Pseudomonas protegens DA1.2, P. chlororaphis 4CH, and metsulfuron-methyl treatment on the fresh weight (FW) of one wheat (A) or rapeseed (B) shoot; significantly different means are indicated by different letters (p < 0.05, n = 30).
Figure 3
Figure 3
Effect of Pseudomonas protegens DA1.2, P. chlororaphis 4CH, and metsulfuron-methyl treatment on the MDA level in wheat (A) and rapeseed (B) shoots; significantly different means are indicated by different letters (p < 0.05, n = 5).
Figure 4
Figure 4
Effect of Pseudomonas protegens DA1.2, P. chlororaphis 4CH, and metsulfuron-methyl treatment on the activity of GR (A), APX (B), SOD (C), and CAT (D) from wheat shoots; significantly different means are indicated by different letters (p < 0.05, n = 6).
Figure 5
Figure 5
Effect of Pseudomonas protegens DA1.2, P. chlororaphis 4CH, and metsulfuron-methyl treatment on the activity of GR (A), APX (B), SOD (C), and CAT (D) from canola shoots; significantly different means are indicated by different letters (p < 0.05, n = 6).
Figure 6
Figure 6
Effect of strains Pseudomonas protegens DA1.2, P. chlororaphis 4CH, and metsulfuron-methyl treatment on the ALS activity in wheat (A) and rapeseed (B) shoots; significantly different means are indicated by different letters (p < 0.05, n = 6).
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