Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Apr;46(2):183-199.
doi: 10.1007/s10529-023-03463-y. Epub 2024 Jan 22.

Isolation of delignifying bacteria and optimization of microbial pretreatment of biomass for bioenergy

B Rabi Prasad  1 , Suman Polaki  2 , Radha Krushna Padhi  3
Affiliations

Isolation of delignifying bacteria and optimization of microbial pretreatment of biomass for bioenergy

B Rabi Prasad et al. Biotechnol Lett. 2024 Apr.

Abstract

Microbial pretreatment of lignocellulosic biomass holds significant promise for environmentally friendly biofuel production, offering an alternative to fossil fuels. This study focused on the isolation and characterization of two novel delignifying bacteria, GIET1 and GIET2, to enhance cellulose accessibility by lignin degradation. Molecular characterization confirmed their genetic identities, providing valuable microbial resources for biofuel production. Our results revealed distinct preferences for temperature, pH, and incubation period for the two bacteria. Bacillus haynesii exhibited optimal performance under moderate conditions and shorter incubation period, making it suitable for rice straw and sugarcane bagasse pretreatment. In contrast, Paenibacillus alvei thrived at higher temperatures and slightly alkaline pH, requiring a longer incubation period ideal for corn stalk pretreatment. These strain-specific requirements highlight the importance of tailoring pretreatment conditions to specific feedstocks. Structural, chemical, and morphological analyses demonstrated that microbial pretreatment reduced the amorphous lignin, increasing cellulose crystallinity and accessibility. These findings underscore the potential of microbial pretreatment to enhance biofuel production by modifying the lignocellulosic biomass. Such environmentally friendly bioconversion processes offer sustainable and cleaner energy solutions. Further research to optimize these methods for scalability and broader application is necessary in the pursuit for more efficient and greener biofuel production.

Keywords: Biofuel; Cellulose accessibility; Hydrolysis; Lignin degradation; Lignocellulosic biomass; Sustainable energy.

PubMed Disclaimer

References

    1. Ahmed E et al (2022) Lignocellulosic biomasses from agricultural wastes improved the quality and physicochemical properties of frying oils. Foods 11(19):3149. https://doi.org/10.3390/foods11193149 - DOI - PubMed - PMC
    1. Baruah J et al (2018) Recent trends in the pretreatment of lignocellulosic biomass for value-added products. Front Energy Res. https://doi.org/10.3389/fenrg.2018.00141 - DOI
    1. Ferdeș M et al (2020) Microorganisms and enzymes used in the biological pretreatment of the substrate to enhance biogas production: a review. Sustainability 12(17):7205. https://doi.org/10.3390/su12177205 - DOI
    1. Gong JSQ et al (2023) Enhanced enzymolysis and bioethanol yield from tobacco stem waste based on mild synergistic pretreatment. Front Energy Res. https://doi.org/10.3389/fenrg.2022.989393 - DOI
    1. Harris D, DeBolt S (2008) Relative crystallinity of plant biomass: studies on assembly, adaptation and acclimation. PLoS ONE 3(8):e2897. https://doi.org/10.1371/journal.pone.0002897 - DOI - PubMed - PMC

LinkOut - more resources