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Review
. 2024 Jan 17;25(2):1142.
doi: 10.3390/ijms25021142.

Advancements in Reference Gene Selection for Fruit Trees: A Comprehensive Review

Shujun Peng  1   2 , Irfan Ali Sabir  2 , Xinglong Hu  1   2 , Jiayi Chen  1   2 , Yonghua Qin  1   2
Affiliations
Review

Advancements in Reference Gene Selection for Fruit Trees: A Comprehensive Review

Shujun Peng et al. Int J Mol Sci. .

Abstract

Real-time quantitative polymerase chain reaction (qRT-PCR) has been widely used in gene expression analyses due to its advantages of sensitivity, accuracy and high throughput. The stability of internal reference genes has progressively emerged as a major factor affecting the precision of qRT-PCR results. However, the stability of the expression of the reference genes needs to be determined further in different cells or organs, physiological and experimental conditions. Methods for evaluating these candidate internal reference genes have also evolved from simple single software evaluation to more reliable and accurate internal reference gene evaluation by combining different software tools in a comprehensive analysis. This study intends to provide a definitive reference for upcoming research that will be conducted on fruit trees. The primary focus of this review is to summarize the research progress in recent years regarding the selection and stability analysis of candidate reference genes for different fruit trees.

Keywords: fruit trees; qRT-PCR; reference genes.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
qRT-PCR illustration model.
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