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Published Erratum
. 2024 Feb 16;19(2):584-585.
doi: 10.1021/acschembio.3c00733. Epub 2024 Jan 22.

Correction to "Analysis of the Sequence Preference of Saporin by Deep Sequencing"

Samuel Hauf, Rachapun Rotrattanadumrong, Yohei Yokobayashi
Published Erratum

Correction to "Analysis of the Sequence Preference of Saporin by Deep Sequencing"

Samuel Hauf et al. ACS Chem Biol. .
No abstract available

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Figures

Figure 5
Figure 5
Distribution of recovery ratios. (A) Shape of the distribution of recovery ratios for all variants. The distributions of the SRL-based libraries are flatter than those of the loop libraries indicative of a more promiscuous activity. (B) Dependence of recovery on the number of adenine residues. An increasing number of adenine residues reduces the recovery ratios of variants, indicating that adenine is targeted nonspecifically. Interestingly, there are some variants in both DNA libraries that exhibit much lower recovery ratios compared to those of the rest of the population. This indicates that some specific sequence pattern is targeted. (C) Minimum free energy structures of two sensitive variants from the SRL DNA library as predicted by NUPACK.33 These variants form secondary hairpins with small loops. The small loops closely resemble the sensitive ACG pattern identified in the DNA loop library.
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