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. 2023 Dec 12;11(1):ofad629.
doi: 10.1093/ofid/ofad629. eCollection 2024 Jan.

Reflex Human Immunodeficiency Virus (HIV) Type 1 RNA Testing Enables Timely Differentiation of False-Positive Results From Acute HIV Infection

Christopher Kaperak  1 , Dylan Eller  2   3 , Samantha A Devlin  2   3 , André Hall  2   3 , Jessica Schmitt  2   3 , Eleanor E Friedman  2   3 , Kathleen G Beavis  4 , Kimberly A Stanford  5 , David Pitrak  2   3 , Moira C McNulty  2   3
Affiliations

Reflex Human Immunodeficiency Virus (HIV) Type 1 RNA Testing Enables Timely Differentiation of False-Positive Results From Acute HIV Infection

Christopher Kaperak et al. Open Forum Infect Dis. .

Abstract

Accurate, timely human immunodeficiency virus (HIV) diagnosis is critical. Routine HIV screening program data were examined before and after reflex HIV type 1 RNA testing. Reflex testing facilitated confirmation of reactive HIV screening assays (as true or false positives) (odds ratio, 23.7 [95% confidence interval, 6.7-83.4]; P < .0001), improving detection of acute HIV and reducing unconfirmed discordant results.

Keywords: HIV testing; acute HIV infection; discordant results; reflex HIV-1 RNA.

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Conflict of interest statement

Potential conflicts of interest. M. C. M. has served on an advisory board for Gilead Sciences, Inc. J. S. and D. P. report grant support from Gilead Sciences, Inc. All other authors report no potential conflicts.

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