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. 2024 Feb;44(1):110-120.
doi: 10.1007/s11596-023-2800-z. Epub 2024 Jan 26.

Inhibition of Bcl-6 Expression Ameliorates Asthmatic Characteristics in Mice

Chang-Zhi Zhou #  1 , Xiong Xiong #  2 , Wei-Jun Tan  1 , Ya-Fei Wang  1 , Zhen Yang  1 , Xue-Ying Li  1 , Xiu-Wen Yang  1 , Xiao-Fan Liu  1 , Sun-Feng Yu  1 , Liang-Chao Wang  3 , Shuang Geng  4
Affiliations

Inhibition of Bcl-6 Expression Ameliorates Asthmatic Characteristics in Mice

Chang-Zhi Zhou et al. Curr Med Sci. 2024 Feb.

Abstract

Objective: The function of Bcl-6 in T follicular helper (Tfh) cell maturation is indispensable, and Tfh cells play a pivotal role in asthma. This study investigated the impact of Bcl-6 on asthmatic traits.

Methods: The microscopic pathological alterations, airway resistance (AR), and lung compliance (LC) were determined in asthmatic mice and Bcl-6 interference mice. The surface molecular markers of Tfh cells and the Bcl-6 mRNA and protein expression were determined by flow cytometry, RT-qPCR, and Western blotting, respectively. The relationships between the Tfh cell ratio and the IgE and IgG1 concentrations in peripheral blood mononuclear cells (PBMCs) and bronchoalveolar lavage fluid (BALF) were determined.

Results: Asthmatic inflammatory changes were observed in the lung tissue and were attenuated by Bcl-6 siRNA and dexamethasone (DXM). Asthmatic mice exhibited an increased AR and a decreased LC, while Bcl-6 siRNA or DXM mitigated these changes. The percentages of Tfh cells and eosinophils were significantly increased in the asthmatic mice, and they significantly decreased after Bcl-6 inhibition or DXM treatment. RT-qPCR and Western blotting analyses revealed that the Bcl-6 expression level in PBMCs was significantly higher in asthmatic mice, and it decreased following Bcl-6 inhibition or DXM treatment. The IgE expression in the serum and BALF and the B cell expression in PBMCs exhibited a similar trend. In asthmatic mice, the ratio of Tfh cells in the peripheral blood showed a strong positive correlation with the IgE levels in the serum and BALF, but not with the IgG1 levels.

Conclusion: The amelioration of airway inflammation and airway hyper-responsiveness is achieved through Bcl-6 suppression, which effectively hinders Tfh cell differentiation, ultimately resulting in a concurrent reduction in IgE production.

Keywords: Bcl-6; Tfh; airway hyper-responsiveness; asthma; mouse model.

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References

    1. Lemanske RF Jr, Busse WW. Asthma: clinical expression and molecular mechanisms. J Allergy Clin Immunol, 2010,125(2 Suppl 2):S95–S102 - DOI - PubMed - PMC
    1. Papi A, Brightling C, Pedersen SE, et al. Asthma. Lancet, 2018,391(10122):783–800 - DOI - PubMed
    1. Balaha MF, Tanaka H, Yamashita H, et al. Oral Nigella sativa oil ameliorates ovalbumin-induced bronchial asthma in mice. Int Immunopharmacol, 2012,14(2):224–231 - DOI - PubMed
    1. Park HJ, Lee CM, Jung ID, et al. Quercetin regulates Th1/Th2 balance in a murine model of asthma. Int Immunopharmacol, 2009,9(3):261–267 - DOI - PubMed
    1. Schaerli P, Willimann K, Lang AB, et al. CXC chemokine receptor 5 expression defines follicular homing T cells with B cell helper function. J Exp Med, 2000,192(11):1553–1562 - DOI - PubMed - PMC