Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1986 Nov 1;239(3):587-92.
doi: 10.1042/bj2390587.

Purification and properties of a xylanase from Streptomyces lividans

R Morosoli, J L Bertrand, F Mondou, F Shareck, D Kluepfel

Purification and properties of a xylanase from Streptomyces lividans

R Morosoli et al. Biochem J. .

Abstract

An extracellular xylanase produced by a cellulase-negative mutant strain of Streptomyces lividans 1326 was purified to homogeneity. The purified enzyme has an apparent Mr of 43,000 and pI of 5.2. The pH and temperature optima for the activity were 6.0 and 60 degrees C respectively, and the Km and Vmax. values, determined with a soluble oat spelts xylan, were 0.78 mg/ml and 0.85 mmol/min per mg of enzyme. The xylanase showed no activity towards CM-cellulose and p-nitrophenyl beta-D-xyloside. The enzyme degraded xylan, producing mainly xylobiose, a mixture of xylo-oligosaccharides and a small amount of xylose as end products. Its pattern of action on beta-1,4-D-xylan indicates that it is a beta-1,4-endoxylanase (EC 3.2.1.8).

PubMed Disclaimer

References

    1. Eur J Biochem. 1982 Jun;124(3):635-42 - PubMed
    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. Appl Environ Microbiol. 1983 Jul;46(1):17-21 - PubMed
    1. Appl Environ Microbiol. 1983 Aug;46(2):511-4 - PubMed
    1. Biochim Biophys Acta. 1972 Jan 26;257(1):11-9 - PubMed

Publication types

MeSH terms