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. 1986 Dec 15;240(3):885-9.
doi: 10.1042/bj2400885.

Tissue distribution of rat glutathione transferase subunit 7, a hepatoma marker

S E Pemble, J B Taylor, B Ketterer

Tissue distribution of rat glutathione transferase subunit 7, a hepatoma marker

S E Pemble et al. Biochem J. .

Abstract

Polyadenylated RNA isolated from NN-dimethyl-4-aminoazobenzene-induced rat hepatoma was used to prepare a cDNA library in lambda gt10. Full-length clones complementary to mRNA coding for glutathione transferase subunit 7 were isolated and one of these clones (pGSTr7) was fully characterized. In Northern blot analysis, mRNA hybridizing to 32P-labelled pGSTr7 was found in poly(A)-containing RNA isolated from seven normal rat tissues but not from testis and liver. A similar hybridizing mRNA species was also detected in human placental mRNA. The same probe, used in a Southern blot analysis of genomic DNA, suggests the presence of a multigene family in the rat.

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