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. 2024 Jan 26;22(1):21.
doi: 10.1186/s12915-023-01802-z.

Organellar electrophysiology: DNA nanodevices charging at the unmeasured

Senthil Arumugam  1   2
Affiliations

Organellar electrophysiology: DNA nanodevices charging at the unmeasured

Senthil Arumugam. BMC Biol. .

Abstract

Organellar ion channels are regulators of key processes of cellular homeostasis as well as being involved in diseases including cancer, neurological disorders and virus infection. Individual organelle-level electrophysiology has until recently only been performed using isolated organelles, removing any direct physiological context. New DNA-based nanodevices — Clensor (chloride sensor), RatiNa (sodium sensor) and pHlicKer (potassium sensor), now allow imaging-based mapping of sodium and potassium levels in membranous organelles. This breakthrough paves the way for studying ion homeostasis relevant to a variety of diseases as well as fundamental processes.

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Conflict of interest statement

The author declares no competing interests.

References

    1. Wang X, Zhang X, Dong XP, Samie M, Li X, Cheng X, et al. TPC proteins are phosphoinositide- activated sodium-selective ion channels in endosomes and lysosomes. Cell. 2012;151(2):372–383. doi: 10.1016/j.cell.2012.08.036. - DOI - PMC - PubMed
    1. Hover S, Foster B, Fontana J, Kohl A, Goldstein SAN, Barr JN, et al. Bunyavirus requirement for endosomal K+ reveals new roles of cellular ion channels during infection. PLoS Pathog. 2018;14(1):e1006845. doi: 10.1371/journal.ppat.1006845. - DOI - PMC - PubMed
    1. Scott CC, Gruenberg J. Ion flux and the function of endosomes and lysosomes: pH is just the start: the flux of ions across endosomal membranes influences endosome function not only through regulation of the luminal pH. BioEssays. 2011;33(2):103–110. doi: 10.1002/bies.201000108. - DOI - PubMed
    1. Neher E, Sakmann B. Single-channel currents recorded from membrane of denervated frog muscle fibres. Nature. 1976;260(5554):799–802. doi: 10.1038/260799a0. - DOI - PubMed
    1. Chen CC, Cang C, Fenske S, Butz E, Chao YK, Biel M, et al. Patch-clamp technique to characterize ion channels in enlarged individual endolysosomes. Nat Protoc. 2017;12(8):1639–1658. doi: 10.1038/nprot.2017.036. - DOI - PubMed

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