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. 2024 Jan 28;51(1):222.
doi: 10.1007/s11033-023-09204-8.

Cardamonin decreases inflammatory mediator expression in IL-1β-stimulated human periodontal ligament cells

Risa Okamoto  1 , Yoshitaka Hosokawa  2 , Ikuko Hosokawa  1 , Kazumi Ozaki  3 , Keiichi Hosaka  1   4
Affiliations

Cardamonin decreases inflammatory mediator expression in IL-1β-stimulated human periodontal ligament cells

Risa Okamoto et al. Mol Biol Rep. .

Abstract

Background: Cardamonin is classified as a natural chalcone, and has been reported to possess various bioactive effects. However, there have been limited attempts to utilize cardamonin in the treatment of periodontitis. This study aimed to investigate whether cardamonin has anti-inflammatory effects on human periodontal ligament cells (HPDLCs), which are a component cell of periodontal tissue. Specifically, the study seeks to determine whether cardamonin affects the expression of inflammatory mediators, such as cytokines and adhesion molecules, induced by interleukin-1β (IL-1β) in HPDLCs, as well as the signaling pathways activated by IL-1β.

Methods: Cytokine and chemokine levels in supernatants of HPDLCs were measured by ELISA. Western blot analysis was used to measure protein expression and signal transduction pathway activation in HPDLCs.

Results: We found that IL-1β-induced CC chemokine ligand (CCL)2, CCL5, CCL20, CXC-chemokine ligand (CXCL)10, and interleukin (IL)-6 production and intercellular adhesion molecule (ICAM)-1 and cyclooxygenase (COX)-2 expression in HPDLCs were suppressed by cardamonin treatment. We also found that cardamonin suppressed IL-1β-activated nuclear factor (NF)-κB pathway, and the phosphorylation of signal transducer and activator of transcription (STAT)3. Furthermore, cardamonin treatment enhanced the expression of the antioxidant enzymes, heme oxygenase (HO)-1 and NAD(P)H dehydrogenase [quinone] 1 (NQO1), in HPDLCs.

Conclusion: In this study, we found that cardamonin could suppress the production of inflammatory mediators in HPDLCs as well as the activation of several signaling pathways induced by IL-1β treatment.

Keywords: Anti-inflammatory effect; Antioxidant enzyme; Cardamonin; Periodontal ligament cells; Signal transduction.

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References

    1. Graves D (2008) Cytokines that promote periodontal tissue destruction. J Periodontol 79:1585–1591 - DOI - PubMed
    1. Ng E, Tay JRH, Boey SK, Laine ML, Ivanovski S, Seneviratne CJ (2023) Antibiotic resistance in the microbiota of periodontitis patients: an update of current findings. Crit Rev Microbiol 4:1–12
    1. Nawaz J, Rasul A, Shah MA, Hussain G, Riaz A, Sarfraz I, Zafar S, Adnan M, Khan AH, Selamoglu Z (2020) Cardamonin: a new player to fight cancer via multiple cancer signaling pathways. Life Sci 250:117591 - DOI - PubMed
    1. Israf DA, Khaizurin TA, Syahida A, Lajis NH, Khozirah S (2007) Cardamonin inhibits COX and iNOS expression via inhibition of p65NF-kappaB nuclear translocation and Ikappa-B phosphorylation in RAW 264.7 macrophage cells. Mol Immunol 44:673–679 - DOI - PubMed
    1. Yu H, Jiang W, Du H, Xing Y, Bai G, Zhang Y, Li Y, Jiang H, Zhang Y, Wang J, Wang P, Bai X (2014) Involvement of the Akt/NF-κB pathways in the HTNV-mediated increase of IL-6, CCL5, ICAM-1, and VCAM-1 in HUVECs. PLoS ONE 9:e93810 - DOI - PubMed - PMC

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