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. 2024 Jan 29;259(2):50.
doi: 10.1007/s00425-023-04282-1.

Functional characterization of genes related to triterpene and flavonoid biosynthesis in Cyclocarya paliurus

Shuang-Yan Zhang #  1   2 , Yu-Qing Peng #  1   2 , Gui-Sheng Xiang  1   2 , Wan-Ling Song  1   2 , Lei Feng  1   2 , Xin-Yue Jiang  1   2 , Xue-Jiao Li  1   2 , Si-Mei He  1   2 , Sheng-Chao Yang  1   2 , Yan Zhao  3   4 , Guang-Hui Zhang  5   6
Affiliations

Functional characterization of genes related to triterpene and flavonoid biosynthesis in Cyclocarya paliurus

Shuang-Yan Zhang et al. Planta. .

Abstract

The oxidosqualene cyclases (OSCs) generating triterpenoid skeletons in Cyclocarya paliurus were identified for the first time, and two uridine diphosphate (UDP)-glycosyltransferases (UGTs) catalyzing the glycosylation of flavonoids were characterized. Cyclocarya paliurus, a native rare dicotyledonous plant in China, contains an abundance of triterpenoid saponins and flavonoid glycosides that exhibit valuable pharmaceutical effects in preventing hypertension, hyperlipidemia, and diabetes. However, the molecular mechanism explaining the biosynthesis of triterpenoid saponin and flavonoid glycoside in C. paliurus remains unclear. In this study, the triterpene content in different tissues and the expression pattern of genes encoding the key enzymes associated with triterpenoid saponin and flavonoid glycoside biosynthesis were studied using transcriptome and metabolome analysis. The eight upstream oxidosqualene cyclases (OSCs) involved in triterpenoid saponin biosynthesis were functionally characterized, among them CpalOSC6 catalyzed 2,3;22,23-dioxidosqualene to form 3-epicabraleadiol; CpalOSC8 cyclized 2,3-oxidosqualene to generate dammarenediol-II; CpalOSC2 and CpalOSC3 produced β-amyrin and CpalOSC4 produced cycloartenol, while CpalOSC2-CpalOSC5, CpalOSC7, and CpalOSC8 all produced lanosterol. However, no catalytic product was detected for CpalOSC1. Moreover, two downstream flavonoid uridine diphosphate (UDP)-glycosyltransferases (UGTs) (CpalUGT015 and CpalUGT100) that catalyze the last step of flavonoid glycoside biosynthesis were functionally elucidated. These results uncovered the key genes involved in the biosynthesis of triterpenoid saponins and flavonoid glycosides in C. paliurus that could be applied to produce flavonoid glycosides and key triterpenoid saponins in the future via a synthetic strategy.

Keywords: Biosynthesis; C. paliurus; Flavonoid glycosides; Oxidosqualene cyclases (OSCs); Triterpenoid saponins; Uridine diphosphate (UDP)-glycosyltransferases (UGTs).

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References

    1. Aramaki T, Blanc-Mathieu R, Endo H et al (2019) KofamKOALA: KEGG ortholog assignment based on profile HMM and adaptive score threshold. Bioinformatics 36(7):2251–2252. https://doi.org/10.1093/bioinformatics/btz859/5631907 - DOI - PMC
    1. Blum M, Chang HY, Chuguransky S et al (2021) The InterPro protein families and domains database: 20 years on. Nucleic Acids Res 49(D1):D344–D354. https://doi.org/10.1093/nar/gkaa977 - DOI - PubMed
    1. Bolger AM, Lohse M, Usadel B (2014) Trimmomatic: A flexible trimmer for Illumina sequence data. Bioinformatics 30:2114–2120. https://doi.org/10.1093/bioinformatics/btu170 - DOI - PubMed - PMC
    1. Bowles D, Isayenkova J, Lim EK, Poppenberger B (2005) Glycosyltransferases: managers of small molecules. Curr Opin Plant Biol 8:254–263. https://doi.org/10.1016/j.pbi.2005.03.007 - DOI - PubMed
    1. Bowles D, Lim EK, Poppenberger B, Vaistij FE (2006) Glycosyltransferases of lipophilic small molecules. Annu Rev Plant Biol 57(1):567–597. https://doi.org/10.1146/annurev.arplant.57.032905.105429 - DOI - PubMed

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