Molecular identification and genetic diversity of zoonotic hookworm infections in domestic dogs from northeastern, Thailand

doi:10.1007/s00436-024-08134-z

. 2024 Jan 29;123(2):115.

doi: 10.1007/s00436-024-08134-z.

Molecular identification and genetic diversity of zoonotic hookworm infections in domestic dogs from northeastern, Thailand

Rutchanee Rodpai^{1

2}, Oranuch Sanpool^{1

2}, Lakkhana Sadaow^{1

2}, Patcharaporn Boonroumkaew^{1

2}, Pewpan M Intapan^{1

2}, Wanchai Maleewong^{1

2}, Manachai Yingklang³, Penchom Janwan⁴, Kotchaphon Vaisusuk⁵, Wasupon Chatan⁶, Supawadee Piratae⁶, Tongjit Thanchomnang⁷

Affiliations

¹ Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
² Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, 40002, Thailand.
³ Faculty of Public Health, Burapha University, Chonburi, 20131, Thailand.
⁴ Department of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat, 80160, Thailand.
⁵ Department of Veterinary Technology and Veterinary Nursing, Faculty of Agricultural Technology, Rajabhat Maha Sarakham University, Maha Sarakham, 44000, Thailand.
⁶ Faculty of Veterinary Sciences, Mahasarakham University, Maha Sarakham, 44000, Thailand.
⁷ Faculty of Medicine, Mahasarakham University, Maha Sarakham, 44000, Thailand. tongjit.t@msu.ac.th.

PMID: 38285129
DOI: 10.1007/s00436-024-08134-z

Molecular identification and genetic diversity of zoonotic hookworm infections in domestic dogs from northeastern, Thailand

Rutchanee Rodpai et al. Parasitol Res. 2024.

. 2024 Jan 29;123(2):115.

doi: 10.1007/s00436-024-08134-z.

Authors

Affiliations

¹ Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
² Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, 40002, Thailand.
³ Faculty of Public Health, Burapha University, Chonburi, 20131, Thailand.
⁴ Department of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat, 80160, Thailand.
⁵ Department of Veterinary Technology and Veterinary Nursing, Faculty of Agricultural Technology, Rajabhat Maha Sarakham University, Maha Sarakham, 44000, Thailand.
⁶ Faculty of Veterinary Sciences, Mahasarakham University, Maha Sarakham, 44000, Thailand.
⁷ Faculty of Medicine, Mahasarakham University, Maha Sarakham, 44000, Thailand. tongjit.t@msu.ac.th.

PMID: 38285129
DOI: 10.1007/s00436-024-08134-z

Abstract

Hookworm infections remain a significant public health concern in tropical and subtropical regions, including Thailand. This study investigated the species and genetic diversity of hookworm infections in domestic dogs from northeastern Thailand. The molecular analysis focused on amplifying and sequencing specific regions of ribosomal RNA genes (ITS1-5.8S-ITS2 region) and the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene in hookworm larvae recovered from 21 domestic dog stool samples. Among 21 larvae (one larva per infected dog) analyzed, 14 had sequences identical to Ancylostoma caninum, and 7 showed sequences almost identical to Ancylostoma ceylanicum. Phylogenetic analysis of cox1 sequences placed A. caninum and A. ceylanicum in separate clades. The median-joining network of A. caninum cox1 sequences from Thailand showed high haplotype diversity and belonged to the same cluster as sequences from Australia while forming separate clusters from those of A. caninum samples from the USA. The available published A. ceylanicum cox1 sequences (n = 33), in combination with seven sequences in the present study, represented 15 haplotypes distributed among three clusters. Interestingly, A. ceylanicum sequences from dogs and humans shared the same haplotypes. These findings are crucial for recognizing the potential for zoonotic transmission, highlighting the necessity for targeted control measures, and increasing awareness among pet owners and healthcare professionals to mitigate the risk of hookworm transmission to humans.

Keywords: Animal; Genetic diversity; Hookworm; Infectious; Molecular identification; Zoonotic.

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Calvopina M, Aguilar-Rodríguez D, DeGroot A, Cevallos W, Lee GO, Lopez A, Nutman TB, Levy K, Eisenberg J, Sears WJ, Cooper PJ. Calvopina M, et al. Pathogens. 2024 Jul 23;13(8):609. doi: 10.3390/pathogens13080609. Pathogens. 2024. PMID: 39204210 Free PMC article.

References

1. Blouin MS (2002) Molecular prospecting for cryptic species of nematodes: mitochondrial DNA versus internal transcribed spacer. Int J Parasitol 32:527–531. https://doi.org/10.1016/s0020-7519(01)00357-5 - DOI
1. Bowman DD, Montgomery SP, Zajac AM, Eberhard ML, Kazacos KR (2010) Hookworms of dogs and cats as agents of cutaneous larva migrans. Trends Parasitol 26:162–167. https://doi.org/10.1016/j.pt.2010.01.005 - DOI
1. Bui KL, Nguyen TH, Duong HD, Nguyen VL, Nguyen TN, Le LA, Cong HM, Tran KT, Le DV, Nagayasu E, Nonaka N, Yoshida A (2021) Ancylostoma ceylanicum infections in humans in Vietnam. Parasitol Int 84:102405. https://doi.org/10.1016/j.parint.2021.102405 - DOI
1. Conlan JV, Khamlome B, Vongxay K, Elliot A, Pallant L, Sripa B, Blacksell SD, Fenwick S, Thompson RC (2012) Soil-transmitted helminthiasis in Laos: a community-wide cross-sectional study of humans and dogs in a mass drug administration environment. Am J Trop Med Hyg 86:624–634. https://doi.org/10.4269/ajtmh.2012.11-0413 - DOI - PMC
1. Croese J, Loukas A, Opdebeeck J, Fairley S, Prociv P (1994) Human enteric infection with canine hookworms. Ann Intern Med 120:369–374. https://doi.org/10.7326/0003-4819-120-5-199403010-00003 - DOI

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[1] Blouin MS (2002) Molecular prospecting for cryptic species of nematodes: mitochondrial DNA versus internal transcribed spacer. Int J Parasitol 32:527–531. https://doi.org/10.1016/s0020-7519(01)00357-5 - DOI

[2] Blouin MS (2002) Molecular prospecting for cryptic species of nematodes: mitochondrial DNA versus internal transcribed spacer. Int J Parasitol 32:527–531. https://doi.org/10.1016/s0020-7519(01)00357-5 - DOI

[3] Bowman DD, Montgomery SP, Zajac AM, Eberhard ML, Kazacos KR (2010) Hookworms of dogs and cats as agents of cutaneous larva migrans. Trends Parasitol 26:162–167. https://doi.org/10.1016/j.pt.2010.01.005 - DOI

[4] Bowman DD, Montgomery SP, Zajac AM, Eberhard ML, Kazacos KR (2010) Hookworms of dogs and cats as agents of cutaneous larva migrans. Trends Parasitol 26:162–167. https://doi.org/10.1016/j.pt.2010.01.005 - DOI

[5] Bui KL, Nguyen TH, Duong HD, Nguyen VL, Nguyen TN, Le LA, Cong HM, Tran KT, Le DV, Nagayasu E, Nonaka N, Yoshida A (2021) Ancylostoma ceylanicum infections in humans in Vietnam. Parasitol Int 84:102405. https://doi.org/10.1016/j.parint.2021.102405 - DOI

[6] Bui KL, Nguyen TH, Duong HD, Nguyen VL, Nguyen TN, Le LA, Cong HM, Tran KT, Le DV, Nagayasu E, Nonaka N, Yoshida A (2021) Ancylostoma ceylanicum infections in humans in Vietnam. Parasitol Int 84:102405. https://doi.org/10.1016/j.parint.2021.102405 - DOI

[7] Conlan JV, Khamlome B, Vongxay K, Elliot A, Pallant L, Sripa B, Blacksell SD, Fenwick S, Thompson RC (2012) Soil-transmitted helminthiasis in Laos: a community-wide cross-sectional study of humans and dogs in a mass drug administration environment. Am J Trop Med Hyg 86:624–634. https://doi.org/10.4269/ajtmh.2012.11-0413 - DOI - PMC

[8] Conlan JV, Khamlome B, Vongxay K, Elliot A, Pallant L, Sripa B, Blacksell SD, Fenwick S, Thompson RC (2012) Soil-transmitted helminthiasis in Laos: a community-wide cross-sectional study of humans and dogs in a mass drug administration environment. Am J Trop Med Hyg 86:624–634. https://doi.org/10.4269/ajtmh.2012.11-0413 - DOI - PMC

[9] Croese J, Loukas A, Opdebeeck J, Fairley S, Prociv P (1994) Human enteric infection with canine hookworms. Ann Intern Med 120:369–374. https://doi.org/10.7326/0003-4819-120-5-199403010-00003 - DOI

[10] Croese J, Loukas A, Opdebeeck J, Fairley S, Prociv P (1994) Human enteric infection with canine hookworms. Ann Intern Med 120:369–374. https://doi.org/10.7326/0003-4819-120-5-199403010-00003 - DOI

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Molecular identification and genetic diversity of zoonotic hookworm infections in domestic dogs from northeastern, Thailand

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Molecular identification and genetic diversity of zoonotic hookworm infections in domestic dogs from northeastern, Thailand

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