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. 2024:2759:3-8.
doi: 10.1007/978-1-0716-3654-1_1.

Temporary Immersion Systems in Plant Micropropagation

Marco A Ramírez-Mosqueda  1 , Carlos A Cruz-Cruz  2
Affiliations

Temporary Immersion Systems in Plant Micropropagation

Marco A Ramírez-Mosqueda et al. Methods Mol Biol. 2024.

Abstract

Temporary immersion systems (TIS) are technological tools that support plant micropropagation. Given their high efficiency in the in vitro propagation of shoots, a current goal is to update the protocols addressing micropropagation in semisolid culture systems to protocols involving TIS. To this end, different parameters have been evaluated, including TIS types and designs, immersion times, immersion frequencies, and volume of medium per explant, among other characteristics. This has resulted in the improved production of propagules of plants of economic interest and the production of physiologically upgraded plants with high percent survival during acclimatization. TIS are specialized culture flasks that provide countless advantages during the commercial micropropagation of plants.

Keywords: Bioreactors; Commercial propagules; In vitro propagation; Mass propagation.

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