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. 2024:2759:69-76.
doi: 10.1007/978-1-0716-3654-1_7.

In Vitro Multiplication of Agave (A. marmorata and A. potatorum) by Temporary Immersion in SETIS™ Bioreactor

María Del Rosario Moreno-Hernández  1 , Eucario Mancilla-Álvarez  1 , Daniel Aguilar-Jiménez  2 , Jericó Jabín Bello-Bello  3
Affiliations

In Vitro Multiplication of Agave (A. marmorata and A. potatorum) by Temporary Immersion in SETIS™ Bioreactor

María Del Rosario Moreno-Hernández et al. Methods Mol Biol. 2024.

Abstract

In Mexico, wild agaves are important for the production of alcoholic beverages known as mezcal and pulque. However, the propagation of agave seeds and pups is not enough to satisfy the national demand. Temporary immersion systems represent an agave micropropagation alternative that reduces the labor force, increases development, and improves the quality of seedlings. The use of the SETIS™ bioreactor in A. marmorata and A. potatorum improves the multiplication rate and allows rooting. Additionally, this bioreactor reduces the culture time, labor force, and reagents needed while maintaining high survival rates during the acclimatization phase.

Keywords: Acclimatization; Bioreactor; Micropropagation; Shoot proliferation.

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