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. 2024:2759:137-147.
doi: 10.1007/978-1-0716-3654-1_13.

Micropropagation of Encyclia cordigera (Kunth) Dressler in Ebb-and-Flow Bioreactor

Obdulia Baltazar-Bernal  1 , Evelia Guadalupe Mora-González  2
Affiliations

Micropropagation of Encyclia cordigera (Kunth) Dressler in Ebb-and-Flow Bioreactor

Obdulia Baltazar-Bernal et al. Methods Mol Biol. 2024.

Abstract

The use of new technologies for micropropagation such as temporary immersion systems (TISs) is important, because it reduces costs by 40% lowering labor, agar and containers. TISs are containers designed for large-scale, semiautomatic production of plants in a liquid medium, which has been used in propagation of commercial orchids. This tool has high potential for application in micropropagation of medicinal and endangered orchids for conservation and commercial purposes. In this chapter, we describe a detailed protocol for propagation and development of Encyclia cordigera to be used in research projects for small-scale production. This protocol comprises all steps from explant preparation to the establishment orchids plantlets.

Keywords: Asymbiotic germination; Fragrant orchid; Gravity immersion bioreactor; Mexican orchid; Orchid plantlets.

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