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Review
. 2024:2759:227-244.
doi: 10.1007/978-1-0716-3654-1_21.

Orchid Micropropagation Using Temporary Immersion Systems: A Review

Obdulia Baltazar-Bernal  1 , Evelia Guadalupe Mora-González  2 , Marco A Ramírez-Mosqueda  3
Affiliations
Review

Orchid Micropropagation Using Temporary Immersion Systems: A Review

Obdulia Baltazar-Bernal et al. Methods Mol Biol. 2024.

Abstract

Temporary immersion systems (TIS) have been used for orchid micropropagation. The main advantage of TIS use for micropropagation is that the explant is periodically immersed in nutrient media, and then, the nutrient solution is drained, which allows the explant tissue to stay in air. The current review resumes the application of TIS in orchid propagation. Fifty-three papers are discussed considering: explant, culture media, TIS bioreactor type, frequency and immersion time, and the TIS effects in acclimatization phase.

Keywords: Immersion frequency; Large-scale production; Orchidaceae; PLBs; Wild orchid.

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