Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review

Optogenetics and Light-Sheet Microscopy

Laura Maddalena  1 , Paolo Pozzi  2 , Nicolò G. Ceffa  1 , Bas van der Hoeven  1 , Elizabeth C. Carroll  1
Eirini Papagiakoumou  1
, editors.
In: All-Optical Methods to Study Neuronal Function [Internet]. New York: Humana; 2023. Chapter 8.
.
Affiliations
Free Books & Documents
Review

Optogenetics and Light-Sheet Microscopy

Laura Maddalena et al.
Free Books & Documents

Excerpt

Light-sheet microscopy is a powerful method for imaging small translucent samples in vivo, owing to its unique combination of fast imaging speeds, large field of view, and low phototoxicity. This chapter briefly reviews state-of-the-art technology for variations of light-sheet microscopy. We review recent examples of optogenetics in combination with light-sheet microscopy and discuss some current bottlenecks and horizons of light sheet in all-optical physiology. We describe how 3-dimensional optogenetics can be added to an home-built light-sheet microscope, including technical notes about choices in microscope configuration to consider depending on the time and length scales of interest.

PubMed Disclaimer

References

    1. Lemon WC, Pulver SR, Höckendorf B, McDole K, Branson K, Freeman J, Keller PJ (2015) Whole-central nervous system functional imaging in larval Drosophila. Nature Communications 6(Article number 7924) - PMC - PubMed
    1. Vanwalleghem GC, Ahrens MB, Scott EK (2018) Integrative whole-brain neuroscience in larval zebrafish. Curr Opin Neurobiol 50:136–145 - PubMed
    1. Loring MD, Thomson EE, Naumann EA (2020) Whole-brain interactions underlying zebrafish behavior. Curr Opin Neurobiol 65:88–99 - PMC - PubMed
    1. Eschbach C, Zlatic M (2020) Useful road maps: studying Drosophila larva’s central nervous system with the help of connectomics. Curr Opin Neurobiol 65:129–137 - PMC - PubMed
    1. Keller PJ, Ahrens MB (2015) Visualizing whole-brain activity and development at the single-cell level using light-sheet microscopy. Neuron 85:462–483 - PubMed

LinkOut - more resources