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. 2024 Mar 12;13(3):e0121723.
doi: 10.1128/mra.01217-23. Epub 2024 Jan 31.

Complete genome sequence of enterococcal phage G01

Emma K Sheriff  1 , Shelby E Andersen  1 , Anushila Chatterjee  1 , Breck A Duerkop  1
Affiliations

Complete genome sequence of enterococcal phage G01

Emma K Sheriff et al. Microbiol Resour Announc. .

Abstract

Here, we report the annotated genome of enterococcal phage G01. The G01 genome is 41,189 bp in length and contains 67 predicted open reading frames. Host range analysis revealed G01 can infect 28.6% (6/21) of Enterococcus faecalis strains tested and appears to not require the enterococcal phage infection protein PIPEF.

Keywords: Enterococcus; bacteriophages; genomics.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Fig 1
Fig 1
(A) The G01 genome contains 67 predicted open reading frames. Annotations are indicated above the open reading frame, if available, and colored by the predicted function. (B) Purified G01 virions were stained with 0.75% uranyl formate and subjected to transmission electron microscopy on a ThermoFisher Tecnai G2 12 BioTwin equipped with a side-mount XR80 (8mp) CCD AMT camera and a low-mount NS15B (15mp) sCMOS AMT camera, with an accelerating voltage of 80 kV. Micrographs of the G01 virion reveal it has siphophage morphology with a tail approximately 169 nm long and a capsid approximately 45 nm in diameter. Measurements reflect analysis of 17 individual virions using ImageJ (26).
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