Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Apr;51(2):89-92.
doi: 10.1111/iji.12656. Epub 2024 Jan 31.

Saliva direct PCR protocol for HLA-DQB1*02 genotyping

Angeles Carrillo  1 , María Jimena Manzur  1 , Maximiliano Juri Ayub  1
Affiliations

Saliva direct PCR protocol for HLA-DQB1*02 genotyping

Angeles Carrillo et al. Int J Immunogenet. 2024 Apr.

Abstract

Celiac disease (CD) is an immune disorder, that is triggered by gluten ingestion in genetically predisposed individuals. The HLA-DQB1*02 allele is the main predisposing genetic factor and a candidate for first-line genotyping screening. We designed and validated a simple, DNA purification-free PCR protocol directly from crude saliva, enabling the detection of the DQB1*02 allele. This assay also distinguishes homozygous from heterozygous carriers. We propose this method for use in mass screening and/or epidemiological studies.

Keywords: autoimmune; disease; human leucocyte antigen; immunogenetics; molecular biology.

PubMed Disclaimer

References

REFERENCES

    1. Capittini, C., De Silvestri, A., Rebuffi, C., Tinelli, C., & Poddighe, D. (2019). Relevance of HLA-DQB1*02 Allele in the Genetic Predisposition of Children with Celiac Disease: Additional Cues from a Meta-Analysis. Medicina (Kaunas, Lithuania), 55(5), 190. https://doi.org/10.3390/medicina55050190
    1. De Silvestri, A., Capittini, C., Poddighe, D., Valsecchi, C., Marseglia, G., Tagliacarne, S. C., Scotti, V., Rebuffi, C., Pasi, A., Martinetti, M., & Tinelli, C. (2018). HLA-DQ genetics in children with celiac disease: a meta-analysis suggesting a two-step genetic screening procedure starting with HLA-DQ beta chains. Pediatric Research, 83(3), 564-572. https://doi.org/10.1038/pr.2017.307
    1. Lindfors, K., Ciacci, C., Kurppa, K., Lundin, K. E. A., Makharia, G. K., Mearin, M. L., Murray, J. A., Verdu, E. F., & Kaukinen, K. (2019). Coeliac disease. Nature Reviews Disease Primers, 5(1), 3. https://doi.org/10.1038/s41572-018-0054-z
    1. Megiorni, F., Mora, B., Bonamico, M., Barbato, M., Nenna, R., Maiella, G., Lulli, P., & Mazzilli, M. C. (2009). HLA-DQ and risk gradient for celiac disease. Human Immunology, 70(1), 55-59. https://doi.org/10.1016/j.humimm.2008.10.018
    1. Poddighe, D. (2019). Relevance of HLA-DQB1*02 allele in predisposing to coeliac disease. International Journal of Immunogenetics, 46(4), 274-275. https://doi.org/10.1111/iji.12427