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Comparative Study
. 1979 Apr 1;179(1):247-9.
doi: 10.1042/bj1790247.

The specificity of some pig and human pepsins towards synthetic peptide substrates

A P Ryle, C A Auffret
Comparative Study

The specificity of some pig and human pepsins towards synthetic peptide substrates

A P Ryle et al. Biochem J. .

Abstract

1. The peptidase activities of pig pepsins A and C and human pepsin and gastricsin were compared. 2. The peptides studied had the general formula A Leu Val-His-B. Hydrolysis at 37 degrees C and pH 2.07 occurred at the amino side of the leucine residue for all the enzymes and all the peptides. 3. When A was Ac-Ala the peptides were hydrolysed under these conditions slowly by pig pepsin C only. 4. Pig pepsin A and human pepsin were unable to hydrolyse the tyrosine-containing peptides under the conditions tested. Gastricsin (human pepsin C) had about one-third of the activity of pig pepsin C with these substrates. 5. The increase in the rate of hydrolysis caused by the extension of the chain by a single alanine residue was most marked for pig pepsin A and human pepsin.

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References

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