Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024;19(11):1449-1460.
doi: 10.2174/011574888X267226231126185532.

Evaluation of Pancreatic β-cell Differentiation Efficiency of Human iPSC Lines for Clinical Use

Ayumi Horikawa  1 , Kyoko Tsuda  1 , Takayoshi Yamamoto  1 , Tatsuo Michiue  1
Affiliations

Evaluation of Pancreatic β-cell Differentiation Efficiency of Human iPSC Lines for Clinical Use

Ayumi Horikawa et al. Curr Stem Cell Res Ther. 2024.

Abstract

Background: Transplantation of pancreatic β-cells generated from human induced pluripotent stem cells (hiPSCs) has great potential as a root treatment for type 1 diabetes. However, their current level of efficiency to differentiate into β-cells is still not at par for clinical use. Previous research has shown that differentiation efficiency varies among human embryonic stem cells and mouse-induced pluripotent stem cell lines. Therefore, selecting a suitable cell line for efficient induction into desired tissues and organs is crucial.

Methods: In this study, we have evaluated the efficiency of 15 hiPSC lines available for clinical use to differentiate into pancreatic β-cells.

Results: Our investigation has revealed induction efficiency to differ among the hiPSC lines, even when derived from the same donor. Among the hiPSC lines tested, the 16A01 cell line exhibited the highest Insulin expression and low Glucagon expression, suggesting that this cell line is suitable for differentiation into β-cells.

Conclusion: Our study has demonstrated the importance of selecting a suitable hiPSC line for effective differentiation into β-cells.

Keywords: diabetes; iPS cells; insulin; pancreas.; regenerative medicine; stem cell; β-cells.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Foster N.C.; Beck R.W.; Miller K.M.; Clements M.A.; Rickels M.R.; DiMeglio L.A.; Maahs D.M.; Tamborlane W.V.; Bergenstal R.; Smith E.; Olson B.A.; Garg S.K.; State of type 1 diabetes management and outcomes from the T1D exchange in 2016–2018. Diabetes Technol Ther 2019,21(2),66-72 - DOI - PubMed
    1. Miller K.M.; Hermann J.; Foster N.; Hofer S.E.; Rickels M.R.; Danne T.; Clements M.A.; Lilienthal E.; Maahs D.M.; Holl R.W.; Weinstock R.; Izquierdo R.; Sheikh U.; Conboy P.; Bulger J.; Bzdick S.; Klingensmith G.; Banion C.; Barker J.; Cain C.; Nadeau K.; Rewers M.; Rewers A.; Slover R.; Steck A.; Wadwa P.; Zeitler P.; Alonso G.; Forlenza G.; Gerard-Gonzalez A.; Green M.; Gross S.; Majidi S.; Messer L.; Reznick-Lipina T.; Simmons E.; Thivener K.; Weber I.; Willi S.; Lipman T.; Kucheruk O.; Minnock P.; Carchidi C.; Grant B.; Olivos D.; DiMeglio L.; Hannon T.; Evans-Molina C.; Hansen D.; Pottorff T.; Woerner S.; Hildinger M.; Hufferd R.; Newnum A.; Purtlebaugh D.; Smith L.; Wendholt K.; Goland R.; Gandica R.; Williams K.; Pollack S.; Casciano E.; Hochberg J.; Uche C.; Lee J.; Gregg B.; Tan M.; Ang L.; Pop-Busui R.; Thomas I.; Dhadphale E.; Dominowski J.; Garrity A.; Leone V.; Plunkett C.; Plunkett B.; Monzavi R.; Cheung C.; Fisher L.; Kim M.; Miyazaki B.; Pitukcheewanont P.; Sandstrom A.; Austin J.; Change N.; Raymond J.; Ichihara B.; Lipton M.; Flores Garcia J.; Garg S.; Michels A.; Garcetti R.; Green M.; Gutin R.; Nadeau K.; Polsky S.; Shah V.; Voelmle M.; Myers L.; Coe G.; Demmitt J.; Garcia Reyes Y.; Giordano D.; Joshee P.; Nease E.; Nguyen N.; Wolfsdorf J.; Quinn M.; Fontanet C.; Mukherjee S.; Bethin K.; Quattrin T.; Majumdar I.; Mastrandrea L.; Gorman E.; House A.; Michalovic S.; Musial W.; Shine B.; Ahmann A.; Castle J.; Joarder F.; Aby-Daniel D.; Guttmann-Bauman I.; Klopfenstein B.; Morimoto V.; Cady N.; Fitch R.; DeFrang D.; Jahnke K.; Patoine C.; Raman V.; Foster C.; Murray M.; Brown T.; Davis C.; Slater H.; Langvardt J.; Bode B.; Boyd J.; Johnson J.; Newton C.; Ownby J.; Hosey R.S.; Rastogi N.; Winslett B.; Hirsch I.; DeSantis A.; Failor R.A.; Greenbaum C.; Trence D.; Trikudanathan S.; Khakpour D.; Thomson P.; Sameshima L.; Tordillos C.; Clements M.; Turpin A.; Babar G.; Broussard J.; Cernich J.; Dileepan K.; Feldt M.; Moore W.; Musick T.; Patton S.; Yan Y.; Tsai S.; Bedard J.; Elrod A.; Hester L.; Beidelschies M.; de la Garza J.; Haith E.; James J.; Ramey E.; Slover J.; Valentine A.; Watkins D.; Whisenhunt M.; Wierson J.; Wilson D.; Buckingham B.; Maahs D.; Prahalad P.; Hsu L.; Kingman R.; Tabatabai I.; Liljenquist D.; Sulik M.; Vance C.; Halford J.; Funke C.; Appiagyei-Dankah Y.; Beltz E.; Moran K.; Starkman H.; Cerame B.; Chin D.; Ebner-Lyon L.; Sabanosh K.; Silverman L.; Wagner C.; Cheruvu S.; Fox M.; Melchionne F.; Bergenstal R.; Madden M.; Martens T.; Criego A.; Powers M.; Carlson A.; Beasley S.; Olson B.; Thomas L.A.; McCann K.; Dunnigan S.; Ashanti C.; Simmons J.; Russell W.; Jaser S.; Kelley J.; Brendle F.; Williams L.; Savin K.; Flowers K.; Williams G.; Hamburger E.; Davis A.; Hammel B.; Cengiz E.; Tamborlane W.; Weyman K.; Van Name M.; Patel N.; Sherr J.; Tichy E.; Steffen A.; Zgorski M.; Carria L.; Finnegan J.; Duran E.; Mehta S.; Katz M.; Laffel L.; Giani E.; Snelgrove R.; Hanono A.; Commissariat P.; Griffith J.; Atkins A.; Harrington K.; Kim K.; Masclans L.; Naik N.; Ambler-Osborn L.; Schultz A.; Cohen C.; Anderson B.; McGill J.; Granados A.; Clifton M.J.; Hurst S.; Kissel S.; Recklein C.; Kruger D.; Bhan A.; Brown T.; Tassopoulos A.; Hailey A.; Remtema H.; Cushman T.; Wintergerst K.; Watson S.; Kingery S.; Rayborn L.; Rush H.; Foster M.; Deuser A.; Rodriguez-Luna M.; Eubanks S.; Rodriguez H.; Bollepalli S.; Smith L.; Shulman D.; Jorgensen E.V.; Eyth E.; Brownstein R.; Rodriguez J.; O’Bria J.; Aleppo-Kacmarek G.; Hahr A.; Molitch M.; Muayed E.; Toft D.; Fulkerson C.; Adelman D.; Massaro E.; Webb K.; Peters A.; Ruelas V.; Harmel M.; Daniels M.; Forghani N.; Flannery T.; Reh C.; Bhangoo A.; Kashmiri H.; Montgomery K.; Trinh L.; Speer H.; Lane K.; Bergenstal R.; Martens T.; Madden M.; Powers M.; Criego A.; Carlson A.; Olson B.; Beasley S.; McCann K.; Thomas L.A.; Miller C.; Ashanti C.; Solorzano C.B.; Puskaric J.; Benjamin R.; Adkins D.; Spruill A.; Williams C.; Tsalikian E.; Tansey M.; Bansl N.; Cabbage J.; Coffey J.; Schatz D.; Clare-Salzler M.; Cusi K.; Fudge B.; Haller M.; Meehan C.; Rohrs H.; Silverstein J.; Walker A.; Albanese-O’Niell A.; Foss S.; Adams J.; Cintron M.; Thomas N.; Gottschalk M.; Newfield R.; Hashiguchi M.; Sparling D.; Tryggested J.; Beck J.; Less J.; Weber L.; Adi S.; Gitelman S.; Sanda S.; Wong J.; McDonnell M.; Mueller M.; Izadi Z.; Mistry S.; Nelson B.; Looper L.; Frost C.; Redondo M.; Lyons S.; Klinepeter S.; Fegan-Bohm K.; Bacha F.; DeSalvo D.; Butler A.; Hilliard M.; Khetani F.; Yulatic R.; Hudson R.; Irvine L.; Zubair S.; Pace C.; Pitrello A.; Levy W.; Njoku C.; Zipf W.; Dyer J.; Lozano R.; Seiple D.; Corven G.; Jaycox M.; Wood J.; Macleish S.; Gubitosi-Klug R.; Adams R.; McGuigan P.; Casey T.; Campbell W.; Kittelsrud J.; Gupta A.; Peterson V.; Libman I.; Diaz A.; Jelley D.; Crowder C.; Greer D.; Crawford J.; Goudeau S.; Pihoker C.; Yi-Frazier J.; Kearns S.; Pascual M.; Loots B.; Beauregard N.; Rickels M.; O’Brien S.; Agarwal S.; Peleckis A.; Dalton-Bakes C.; Markmann E.; Umpierrez G.; Muir A.; Ramos C.; Behbahani K.; Dhruv N.; Gartzman N.; Nathan B.; Bellin M.; Sunni M.; Flaherty N.; Leschyshyn J.; Schmid K.; Weingartner D.; Ludwig M.; Nelson B.; Kogler A.; Bartyzal A.; Street A.; Pappenfus B.; Sweet J.; Buse J.; Young L.; Bergamo K.; Goley A.; Kirkman M.; Diner J.; Kass A.; Dezube M.; Arnold K.; Evans T.; Sellers S.; Blackman S.; Abel K-L.; Rasbach L.; Ali O.; Wolfgram P.; Fiallo-Sharer R.; Kramer J.; Beesley C.; Bingham-Tyson C.; Unteutsh R.; Harlan D.; Lee M.; Soyka L.; Feldman P.; Thompson M.; Gallagher-Dorval K.; Hubacz L.; Hartigan C.; Ciccarelli C.; Edelen R.; Edelen M.; Borgwadt T.; Stauffacher K.; DeGrote K.; Gruetzmacher C.; Shepperd M.; Bhargava A.; Wright D.; Fitzgerald K.; Khoo T.; Young N.; Borg L.; Stifel K.; Rail C.; Casas L.; Eidenshink E.; Huber C.; Rieder A.; Tuchscherer A.; Broadbent M.; Dolan L.; Corathers S.; Kichler J.; Sheanon N.; Baugh H.; Standiford D.; Weis T.; Fox C.; Schultz C.; Ritter A.; Vendrame F.; Blashke C.; Matheson D.; Sanders-Branca N.; Rudolph J.; Biersdorf D.; Fitch-Danielson J.; Eckerle-Mize D.; Brendle F.; Fry J.; Davis D.; Lovell C.; Hammel B.; Williams L.; Hoffman R.; Chaudhari M.; Kamboj M.; Carr L.; Casas L.; Blehm J.; Tello A.; Walter J.A.; Ward R.; Broadbent M.; Blomquist G.; Stewart M.; Cross P.; Racki S.; Sterchi L.; Gouine D.; Kiesow B.; Welch S.; Philis-Tsimikas A.; Daily G.; Chang A.; McCallum J.; Garcia I.; Vela T.; Loupasi I.; Rosal R.; Toschi E.; Middelbeek R.; Munshi M.; Slyne C.; Atakov-Castillo A.; Fox L.; Mauras N.; Wasserman R.; Damaso L.; Englert K.; Sikes K.; Ponthieux K.; Phillipson L.; Cohen A.; Gannon G.; Deeb L.; Shiver A.; Schroeder L.; Schworm W.; Graham K.; Levy C.; Lam D.; Burtman E.; Levister C.; Ogyaadu S.; Gassner H.; Duke J.; Touger L.; Newbern D.; Hoekstra F.; Harwood K.; Prasad V.; Daguanno J.A.; Pratley R.; Corbin K.; Wright M.; Nagel S.; Water N.; Ghere M.; Whitaker K.; Heptulla R.; Katikaneni R.; Johnson-Newell D.; Crandall J.; Powell D.; Anghel V.; Ghanny S.; Aisenberg J.; Chartoff A.; Sivitz J.; Mathus S.; Cospito T-L.; Thailkill K.; Fowlkes J.; Kalaitzoglou E.; Morales Pozzo A.; Edwards K.; Longitudinal changes in continuous glucose monitoring use among individuals with type 1 Diabetes: International comparison in the German and Austrian DPV and U.S. T1D exchange registries. Diabetes Care 2020,43(1),e1-e2 - DOI - PubMed
    1. Posselt A.M.; Szot G.L.; Frassetto L.A.; Masharani U.; Tavakol M.; Amin R.; McElroy J.; Ramos M.D.; Kerlan R.K.; Fong L.; Vincenti F.; Bluestone J.A.; Stock P.G.; Islet transplantation in type 1 diabetic patients using calcineurin inhibitor-free immunosuppressive protocols based on T-cell adhesion or costimulation blockade. Transplantation 2010,90(12),1595-1601 - DOI - PubMed
    1. Barton F.B.; Rickels M.R.; Alejandro R.; Hering B.J.; Wease S.; Naziruddin B.; Oberholzer J.; Odorico J.S.; Garfinkel M.R.; Levy M.; Pattou F.; Berney T.; Secchi A.; Messinger S.; Senior P.A.; Maffi P.; Posselt A.; Stock P.G.; Kaufman D.B.; Luo X.; Kandeel F.; Cagliero E.; Turgeon N.A.; Witkowski P.; Naji A.; O’Connell P.J.; Greenbaum C.; Kudva Y.C.; Brayman K.L.; Aull M.J.; Larsen C.; Kay T.W.H.; Fernandez L.A.; Vantyghem M.C.; Bellin M.; Shapiro A.M.J.; Improvement in outcomes of clinical islet transplantation: 1999-2010. Diabetes Care 2012,35(7),1436-1445 - DOI - PubMed
    1. Russ H.A.; Parent A.V.; Ringler J.J.; Hennings T.G.; Nair G.G.; Shveygert M.; Guo T.; Puri S.; Haataja L.; Cirulli V.; Blelloch R.; Szot G.L.; Arvan P.; Hebrok M.; Controlled induction of human pancreatic progenitors produces functional beta-like cells in vitro. EMBO J 2015,34(13),1759-1772 - DOI - PubMed

LinkOut - more resources