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. 2024:2762:231-250.
doi: 10.1007/978-1-0716-3666-4_14.

Targeted Glycoproteomics Analysis Using MRM/PRM Approaches

Cristian D Gutierrez Reyes  1 , Akeem Sanni  1 , Moyinoluwa Adeniyi  1 , Damir Mogut  2 , Hector R Najera Gonzalez  3 , Parisa Ahmadi  1 , Mojgan Atashi  1 , Sherifdeen Onigbinde  1 , Yehia Mechref  4
Affiliations

Targeted Glycoproteomics Analysis Using MRM/PRM Approaches

Cristian D Gutierrez Reyes et al. Methods Mol Biol. 2024.

Abstract

MS-target analyses are frequently utilized to analyze and validate structural changes of biomolecules across diverse fields of study such as proteomics, glycoproteomics, glycomics, lipidomics, and metabolomics. Targeted studies are commonly conducted using multiple reaction monitoring (MRM) and parallel reaction monitoring (PRM) techniques. A reliable glycoproteomics analysis in intricate biological matrices is possible with these techniques, which streamline the analytical workflow, lower background interference, and enhance selectivity and specificity.

Keywords: Glycopeptides; MRM; PRM.

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Figures

Figure 1.
Figure 1.
Analytical workflow. A) Sample preparation and B) MRM quantitation of glycopeptides, and C) PRM quantitation of glycopeptides. Glycan nomenclature: Fucose formula image; N-acetylglucosamine formula image; mannose formula image; galactose formula image; sialic acid formula image.
Figure 2.
Figure 2.
Representative CID mass spectra of the N-glycopeptide NGSLFAFR + 4–5-0–2 derived from HBS vitronectin, with a NCE of 35%. Glycan nomenclature as in Figure 1. Figure reproduced from E. Song et. al 2012 [14].
Figure 3.
Figure 3.
Representative HCD mass spectra of the serum fetuin N-glycopeptide LCPDCPLLAPLNDSR + 4–5-0–2 acquired in A) Q Exactive HF, B) Tribrid Fusion Lumos, and C) Exploris 240, with a NCE of 25%. Glycan nomenclature as in Figure 1.
See this image and copyright information in PMC

References

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