Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Jul;12(2):332-339.
doi: 10.61186/rbmb.12.2.332.

The Ratio of Cysteine-Rich Angiogenic Inducer 61 to MicroRNA -155 Expression as a Preeclampsia Diagnostic Marker and Predictor of Its Severity

Sarah Mamdouh Shoeib  1 , Doaa Elwy Abdeldaim  2 , Shaimaa Samir Mashal  3 , Rowida Raafat Ibrahim  4 , Lamees Mohamed Dawood  4 , Doaa Shatat  5 , Yasmine Ibrahim El-Masry  5 , Ahmed Almeldin  6 , Radwa Mahamoud El Sharaby  1
Affiliations

The Ratio of Cysteine-Rich Angiogenic Inducer 61 to MicroRNA -155 Expression as a Preeclampsia Diagnostic Marker and Predictor of Its Severity

Sarah Mamdouh Shoeib et al. Rep Biochem Mol Biol. 2023 Jul.

Abstract

Background: Preeclampsia (PE) is a multisystem pregnancy disorder that increases maternal-perinatal morbidity and mortality significantly. MicroRNA-155 (miR-155) overexpression in the sera of pregnant women has been linked to preeclampsia. Researchers discovered that miR-155 acts during pregnancy by down-regulating and reducing the cysteine-rich angiogenic inducer 61 (CYR61), which causes local ischemia as well as oxidative stress.

Methods: The level of miR-155 expression in all serum samples was quantified using real-time polymerase chain reaction (RT-PCR), and serum CYR61 was measured using enzyme-linked immunosorbent assays. Together with the Cyr-61/miR-155 ratio, they were evaluated as biomarkers for PE pathogenesis and severity prediction.

Results: MiR-155 expression, serum CYR61 levels, and Cyr-61/miR-155 ratios were all significantly higher in PE patients compared to the control group. Serum CYR61 levels and the Cyr-61/miR-155 ratio differed significantly between mild and severe PE patients.

Conclusions: MiR-155 expression, serum CYR61 levels, and Cyr-61/miR-155 may serve as biomarkers for PE pathogenesis and severity prediction.

Keywords: Biomarker; Cysteine-Rich Protein 61; MicroRNAs; Pre-Eclampsia.

PubMed Disclaimer

Conflict of interest statement

The authors have no relevant financial or non-financial interests to disclose.

Figures

Fig. 1
Fig. 1
Correlation between cyr61 and miR-155 expression.
Fig. 2
Fig. 2
A: miR-155 expression between PE pregnancies and healthy pregnancies; 2.B: Serum level of CYR-61 between PE pregnancies and healthy pregnancies, and 2.C: CYR-61/ miR-155 ratio between PE pregnancies and healthy pregnancies.
Fig. 3
Fig. 3
A: ROC curve analysis for miRNA 155, CYR-61 and CYR-61 ratio between the cases of PE pregnancies and healthy pregnancies; 3.B: ROC curve analysis for miRNA 155, CYR-61 and CYR-61 ratio between mild and severe PE pregnancies.
Fig. 4
Fig. 4
A: miR-155 expression between mild and severe PE pregnancies; 4.B: Serum level of CYR-61 between mild and severe PE pregnancies, and 4.C: CYR-61/ miR-155 ratio between mild and severe PE pregnancies. PE: Preeclampsia.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Phipps EA, Thadhani R, Benzing T, Karumanchi SA. Pre-eclampsia: pathogenesis, novel diagnostics and therapies. Nat Rev Nephrol. 2019;15(5):275–289. - PMC - PubMed
    1. Gathiram P, Moodley J. Pre-eclampsia: its pathogenesis and pathophysiolgy. Cardiovasc J Afr. 2016;27(2):71–8. - PMC - PubMed
    1. Koushki M, Amiri Dash Atan N, Omidi-Ardali H, Rezaei Tavirani M. Assessment of Correlation Between miR-210 Expression and Pre-Eclampsia Risk: A Meta-Analysis. Rep Biochem Mol Biol. 2018;7(1):94–101. - PMC - PubMed
    1. Zhang Y, Diao Z, Su L, Sun H, Li R, Cui H, Hu Y. MicroRNA-155 contributes to preeclampsia by down-regulating CYR61. Am J Obstet Gynecol. 2010;202(5):466. - PubMed
    1. Al-Kawaz HS, Yasser OM, Mousa MJ. A New Method to Estimate Inhibition Percentage of Endogenous Digitalis in Patients with Pre-eclampsia. Rep Biochem Mol Biol. 2022;11(1):138–145. - PMC - PubMed

LinkOut - more resources