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. 2024 Feb 5:39:e390924.
doi: 10.1590/acb390924. eCollection 2024.

Bone protective effect of sinomenine against monosodium iodoacetate induced knee and hip injury in rat model: an inflammatory pathway

Yi-Hao Lei  1 , Xing-Xi Hu  1 , Hong-Jie Wen  1 , Yong-Cheng Deng  1 , Jun-Liang Jiang  1 , Qing-Gang Zhao  1
Affiliations

Bone protective effect of sinomenine against monosodium iodoacetate induced knee and hip injury in rat model: an inflammatory pathway

Yi-Hao Lei et al. Acta Cir Bras. .

Abstract

Purpose: Osteoarthritis (OA) is a degenerative joint disease which is categorized via destruction of joint cartilage and it also affects the various joints, especially knees and hips. Sinomenine active phytoconstituents isolated from the stem of Sinomenium acutum and already proof anti-inflammatory effect against the arthritis model of rodent. In this experimental protocol, we scrutinized the anti-osteoarthritis effect of sinomenine against monosodium iodoacetate (MIA) induced OA in rats.

Methods: MIA (3 mg/50 μL) was used for inducing the OA in the rats, and rats received the oral administration of sinomenine (2.5, 5 and 7.5 mg/kg body weight) up to the end of the experimental study (four weeks). The body and organs weight were estimated. Aggrecan, C-terminal cross-linked telopeptide of type II collagen (CTX-II), glycosaminoglycans (GCGs), monocyte chemoattractant protein-1 (MCP-1), Interferon gamma (IFN-γ), antioxidant, inflammatory cytokines, inflammatory mediators and matrix metalloproteinases (MMP) were analyzed.

Results: Sinomenine significantly (P < 0.001) boosted the body weight and reduced the heart weight, but the weight of spleen and kidney remain unchanged. Sinomenine significantly (P < 0.001) reduced the level of nitric oxide, MCP-1 and improved the level of aggrecan, IFN-γ and GCGs. Sinomenine remarkably upregulated the level of glutathione, superoxide dismutase and suppressed the level of malonaldehyde. It effectually modulated the level of inflammatory cytokines and inflammatory mediators and significantly (P < 0.001) reduced the level of MMPs, like MMP-1, 2, 3, 9 and 13.

Conclusions: Sinomenine is a beneficial active agent for the treatment of OA disease.

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Conflict of interest statement

Conflict of interest: Nothing to declare.

Figures

Figure 1
Figure 1. The effect of SM on the body and organ weight of MIA induced OA group rats. (a) body weight, (b) heart weight, (c) spleen weight and (d) kidney weight. All the results are presented as mean ± SD (n = 10 for each group) from three independent experiments.
Figure 2
Figure 2. Effect of SM on the nitric oxide of MIA induced OA group rats. All the results are presented as mean ± SD (n = 10 for each group) from three independent experiments.
Figure 3
Figure 3. Effect of SN on the aggrecan and GAGs of MIA induced OA group rats. (a) aggrecan and (b) GAGs. All the results are presented as mean ± SD (n = 10 for each group) from three independent experiments.
Figure 4
Figure 4. Effect of SM on the INF-γ and MCP-1 of MIA induced OA group rats. (a) INF-γ and (b) MCP-1. All the results are presented as mean ± SD (n=10 for each group) from three independent experiments.
Figure 5
Figure 5. Effect of SM on the antioxidant parameters of MIA induced OA group rats. (a) GSH, (b) SOD and (c) MDA. All the results are presented as mean ± SD (n = 10 for each group) from three independent experiments.
Figure 6
Figure 6. Effect of SM on the inflammatory cytokines of MIA induced OA group rats. (a) TNF-α, (b) IL-1β, (c) IL-6 and (d) IL-10. All the results are presented as mean ± SD (n = 10 for each group) from three independent experiments.
Figure 7
Figure 7. Effect of SM on the inflammatory parameters of MIA induced OA group rats. (a) CTX-II, (b) PGE2, (c) COX-2 and (d) iNOS. All the results are presented as mean ± SD (n = 10 for each group) from three independent experiments.
Figure 8
Figure 8. Effect of sinomenine on the MMPs parameters of MIA induced OA group rats. (a) MMP-1, (b) MMP-2, (c) MMP-3, (d) MMP-9 and (e) MMP-13. All the results are presented as mean ± SD (n = 10 for each group) from three independent experiments.
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