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. 2023 Mar 15:11:e98162.
doi: 10.3897/BDJ.11.e98162. eCollection 2023.

The gut microbiota diversity of five Orthoptera (Insecta, Polyneoptera) insects determined by DNA metabarcoding

Yantong Liu  1 , Lina Zhao  2 , Zhongying Qiu  1 , Hao Yuan  1
Affiliations

The gut microbiota diversity of five Orthoptera (Insecta, Polyneoptera) insects determined by DNA metabarcoding

Yantong Liu et al. Biodivers Data J. .

Abstract

Most orthopteran insects are phytophagous and some are important pests in agriculture and forests. Many intestinal microflora of Orthoptera insects have been reported, primarily from Acridoidea and there have been few reports of other taxa. In this study, we collected 15 individuals representing five species (Ruspolialineosa, Tetrixjaponica, Erianthusversicolor, Gryllotalpaorientalis and Teleogryllusemma) belonging to five orthopteran superfamilies (Tettigonioidea, Tetrigoidea, Eumastacoidea, Gryllotalpoidea and Grylloidea) to characterise and compare the gut microbiota with greater taxonomic width by performing sequencing analysis of the 16S rRNA V4 region in gut material. A total of 606,053 high-quality sequences and 3,105 OTUs were acquired from 15 gut samples representing 24 phyla, 48 classes, 69 orders, 133 families and 219 genera. Firmicutes and bacteria were the most abundant phyla, followed by Bacteroidetes, Cyanobacteria, Actinobacteria and Acidobacteria. At the genus level, Serratia, Citrobacter, Wolbachia, Lactobacillus and Parabacteroides were the most predominant genera in R.lineosa, T.japonica, E.versicolor, G.orientalis and T.emma, respectively. Both Principal Coordinates Analysis (PCoA) and heatmap results revealed significant differences in bacterial community composition across species. Additionally, alpha diversity analysis indicated the bacterial richness was significantly different amongst the five species.

Keywords: DNA metabarcoding; Orthoptera; biodiversity; gut microbiota.

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Figures

Figure 1.
Figure 1.
Venn diagram depicting the number of shared and exclusive bacterial OTUs in the bacterial community of five groups. Z: Tetrixjaponica; ZS: Ruspolialineosa; M: Erianthusversicolor; LG: Gryllotalpaorientalis; HLYHL: Teleogryllusemma.
Figure 2.
Figure 2.
Dendrogram and heatmap of bacterial distributions of the top 100 abundant OTUs present in the microbial community of the fifteen samples. The numbers indicate the actual reads number of the OTU. The heatmap plot depicted the relative abundance of each sample and the relative values for OTUs are indicated by colour intensity. Z: Tetrixjaponica; ZS: Ruspolialineosa; M: Erianthusversicolor; LG: Gryllotalpaorientalis; HLYHL: Teleogryllusemma.
Figure 3.
Figure 3.
PCoA plot based on an unweighted UniFrac distance matrix depicting differences in the composition of the gut microbiota of the five groups. In the unweighted UniFrac analysis of the gut samples, the first principal coordinate, explained 40.11% of sample variation and separated groups of LG and HLYHL from others. The third principal coordinate (7.49% of sample variation) separated groups (M) from others. Z: Tetrixjaponica; ZS: Ruspolialineosa; M: Erianthusversicolor; LG: Gryllotalpaorientalis; HLYHL: Teleogryllusemma.
Figure 4.
Figure 4.
Distribution of the gut microbiota composition. A Five groups at phylum level; B Five groups at genus level.
Figure 5.
Figure 5.
The relative abundance (% of individual taxonomic group) of Acidobacteria and bacteria present in the microbial community of the different groups. Differences were analysed by employing ANOVA analysis and Tukey Post Hoc HSD Significance Test (* P < 0.05, ** P < 0.01, *** P < 0.001). Z: Tetrixjaponica; ZS: Ruspolialineosa; M: Erianthusversicolor; LG: Gryllotalpaorientalis; HLYHL: Teleogryllusemma.
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