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. 2024 Feb 8;51(1):289.
doi: 10.1007/s11033-023-09065-1.

Detection of SARS-CoV-2 spike protein D614G mutation using μTGGE

Kevin Maafu Juma  1 , Kenta Morimoto  1 , Vishnu Sharma  2   3 , Kirti Sharma  2 , Radhika Biyani  2 , Manish Biyani  4   5   6 , Teisuke Takita  1 , Kiyoshi Yasukawa  7
Affiliations

Detection of SARS-CoV-2 spike protein D614G mutation using μTGGE

Kevin Maafu Juma et al. Mol Biol Rep. .

Erratum in

Abstract

Background: The accurate and expeditious detection of SARS-CoV-2 mutations is critical for monitoring viral evolution, assessing its impact on transmission, virulence, and vaccine efficacy, and formulating public health interventions. In this study, a detection system utilizing micro temperature gradient gel electrophoresis (μTGGE) was developed for the identification of the D614 and G614 variants of the SARS-CoV-2 spike protein.

Methods: The in vitro synthesized D614 and G614 gene fragments of the SARS-CoV-2 spike protein were amplified via polymerase chain reaction and subjected to μTGGE analysis.

Results: The migration patterns exhibited by the D614 and G614 variants on the polyacrylamide gel were distinctly dissimilar and readily discernible by μTGGE. In particular, the mid-melting pattern of D614 was shorter than that of G614.

Conclusions: Our results demonstrate the capability of μTGGE for the rapid, precise, and cost-effective detection of SARS-CoV-2 spike protein D614 and G614 variants without the need for sequencing. Therefore, this approach holds considerable potential for use in point-of-care mutation assays for SARS-CoV-2 and other pathogens.

Keywords: D614G mutation; Melting pattern; Melting temperature; Micro temperature gradient gel electrophoresis; Mutation detection; SARS-CoV-2 spike protein.

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