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Published Erratum
. 2024 Feb 10;13(1):3.
doi: 10.1186/s13619-023-00185-4.

Correction: RhoA/Rock activation represents a new mechanism for inactivating Wnt/β-catenin signaling in the aging-associated bone loss

Wei Shi #  1   2 , Chengyun Xu #  1   3 , Ying Gong  1 , Jirong Wang  1 , Qianlei Ren  4 , Ziyi Yan  1 , Liu Mei  1 , Chao Tang  1 , Xing Ji  1   5 , Xinhua Hu  1 , Meiyu Qv  1 , Musaddique Hussain  1 , Ling-Hui Zeng  6 , Ximei Wu  7   8
Affiliations
Published Erratum

Correction: RhoA/Rock activation represents a new mechanism for inactivating Wnt/β-catenin signaling in the aging-associated bone loss

Wei Shi et al. Cell Regen. .
No abstract available

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Figures

Fig. 1
Fig. 1
RhoA/Rock constraints Wnt/β-catenin signaling and osteoblastic differentiation. a-c RhoA activation assays in primary murine calvarial osteoblasts (PMCOBs) stimulated with rWnt3a at 100 ng/ml or the indicated concentrations for the indicated times or 60 min in the presence or absence of recombinant Dkk1 (rDkk1) at 100 ng/ml. d,e Western analyses of β-catenin in cytosolic and nuclear fractions of PMCOBs with the indicated genotypes of Col1-Cre (Cre), Col1-Cre;caRhoA + / − (Cre;caRhoA + / −) or Col1-Cre;dnRhoA + / − (Cre;dnRhoA + / −), and in the presence or absence of rWnt3a for 3 h. f Western analyses of β-catenin (β-cat) in cytosolic and nuclear fractions of PMCOBs treated with or without Fasudil at 20 μM and stimulated with or without rWnt3a for 3 h. g-i Alp activity and mineralization nodule formation assays and their quantification in PMCOBs with the indicated genotypes and stimulated with or without rWnt3a at 100 ng/ml for 48 h and 21 d, respectively. Mean ± SEM, *p < 0.05, **,++ p < 0.01, n = 4, Tukey–Kramer multiple comparisons test
Fig. 3
Fig. 3
RhoA/Rock activates Jak1/2 and Gsk3β to destabilize β-catenin. a-c Western analyses in C3H10T1/2 cells transfected with or without RhoA-si or Rock1 + Rock2 siRNA (Rock1,2-si) and treated with or without rWnt3a at 100 ng/ml for the indicated time or 1 h. d, e Western or Lef1-luciferase expression analyses in C3H10T1/2 cells transfected with Gsk3β variants and treated with rWnt3a for 6 or 48 h, respectively. f-k Western analyses in C3H10T1/2 cells transfected with RhoA-si, caRhoA, caRock2, caJak1/2, infected with lentiviral Jak2-shRNA (Jak2-sh), or treated with P6 at 50 nM, followed by incubation with rWnt3a for the indicated times or 1 h. l Co-immunoprecipitation by using IgG1 or Gsk3β antibody in 293 cells transfected with HA-Jak1/2 and Myc-Gsk3β. m In vitro phosphorylation of GSK3β protein by active JAK2 in kinase assay buffer with or without ATP. Phosphorylated proteins were normalized to their total amounts, respectively. Mean ± SD, *, ¶ p < 0.05, **,++ p < 0.01, n = 4, Tukey–Kramer multiple comparisons test
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Erratum for

References

    1. Shi W, Xu C, Gong Y, et al. RhoA/Rock activation represents a new mechanism for inactivating Wnt/β-catenin signaling in the aging-associated bone loss. Cell Regen. 2021;10:8. doi: 10.1186/s13619-020-00071-3. - DOI - PMC - PubMed

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