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. 2024 Jan 24;16(3):317.
doi: 10.3390/polym16030317.

Antimicrobial Antioxidant Polymer Films with Green Silver Nanoparticles from Symphyti radix

Aiste Balciunaitiene  1 , Viktorija Januskevice  1 , Sandra Saunoriute  2 , Urte Raubyte  3 , Jonas Viskelis  1 , Patrick B Memvanga  4 , Pranas Viskelis  1
Affiliations

Antimicrobial Antioxidant Polymer Films with Green Silver Nanoparticles from Symphyti radix

Aiste Balciunaitiene et al. Polymers (Basel). .

Abstract

Antimicrobial natural polymer film with silver nanoparticles (AgNPs) biosynthesized using aqueous plant root extracts as reducing capping agents and for film formatting show extensive applicability for pathogenic microorganism problems. The formation of AgNPs was confirmed by transmission electron microscopy (TEM) and scanning electron microscopy-energy-dispersive spectroscopy (SEM-EDS) techniques. The antimicrobial activity of biofilm with green AgNPs was analysed by inhibiting the growth of Gram-negative and Gram-positive bacteria culture using the Kirby-Bauer disk diffusion susceptibility test. Total phenolic content and antioxidant activity were slightly higher in aqueous extracts of Sym. Radix than in Sym. Radix/AgNPs. The antimicrobial effect of polymer film/AgNPs against selected test bacteria cultures was substantially more robust than with pure film. Pictures of AgNPs obtained by TEM revealed the presence of spherical-shaped nano-objects with an average size 27.45 nm. SEM-EDS studies confirmed the uniform distribution of metal nanoparticles throughout the biopolymeric matrix. Morphological studies of the surface showed that the obtained surface of the films was even, without holes or other relief irregularities. These apparent Symphyti radix polymer film/AgNPs' biological functions could provide a platform for fighting pathogenic bacteria in the era of multi-drug resistance.

Keywords: Symphyti radix; antibacterial activity; antioxidant activity; green synthesis; phytochemical analysis; silver nanoparticles.

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Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
Total phenolic content and in vitro antioxidant capacity using ABTS, DPPH, and FRAP assays of Sym. Radix and Sym. Radix/AgNPs extracts. Values were expressed as mean ± standard deviation (n = 3); different letters indicate statistically significant differences between plant extracts (one-way ANOVA and Tukey’s HSD test, p < 0.05).
Figure 2
Figure 2
SEM images and EDS spectra and element mapping of pure Sym. Radix film.
Figure 3
Figure 3
SEM images and EDS spectra and element mapping of Sym. Radix/AgNPs1 films.
Figure 4
Figure 4
SEM images and EDS spectra and element mapping of Sym. Radix/AgNPs2 films.
Figure 5
Figure 5
SEM images and EDS spectra and element mapping of Sym. Radix/AgNPs3 films.
Figure 6
Figure 6
SEM images and EDS spectra and element mapping of Sym. Radix/AgNPs4 films.
Figure 7
Figure 7
TEM images of biosynthesized Sym. Radix/AgNPs-3 (a,b) in different magnifications and histogram (c).
Figure 8
Figure 8
Changes in the color of Sym. Radix pure films and with AgNPs.
Figure 9
Figure 9
UV-vis absorption spectra of plant extracts and biosynthesized AgNPs.
See this image and copyright information in PMC

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