Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Jan 29;16(3):366.
doi: 10.3390/polym16030366.

Electrochemical Analysis of Curcumin in Real Samples Using Intelligent Materials

Eduardo Jara-Cornejo  1 , Erick Peña-Bedón  1 , Mahely Torres Moya  1 , Sergio Espinoza-Torres  1 , Maria D P T Sotomayor  2   3 , Gino Picasso  1 , Juan C Tuesta  4 , Rosario López  1 , Sabir Khan  1   5   6
Affiliations

Electrochemical Analysis of Curcumin in Real Samples Using Intelligent Materials

Eduardo Jara-Cornejo et al. Polymers (Basel). .

Abstract

Curcumin is a compound of great importance in the food industry due to its biological and pharmacological properties, which include being an antioxidant, anti-inflammatory, antibacterial, antiviral, and anticarcinogenic. This paper proposes the synthesis of an electrochemical sensor based on molecularly imprinted polymers (MIPs) and MWCNT by drop casting deposited on a glassy carbon electrode (GCE) for the selective quantification of curcumin in food samples. The synthesized compounds are characterized by Fourier transform infrared (IR), Brunauer-Emmett-Teller (BET), and electrochemical techniques such as cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The optimal conditions for further experiments were determined by selecting these parameters. We examined three food products, commercial capsules, turmeric rhizomes, and commercial turmeric powder, employing both electrochemical and HPLC methods for the analysis. The electrochemical method revealed a limit of detection (LOD) value of 0.1365 µmol L-1, compared with the HPLC analysis, which gave a value of 3.55 µmol L-1. Furthermore, the MIP material demonstrated superior selectivity for the analyte compared to potential interferents. The recovery percentage, determined using the HPLC method, fell within the range of 87.5% to 102.6.

Keywords: curcumin; electrochemical sensor; molecularly imprinted polymers (MIPs).

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
(a) Molecular imprinted polymer (MIP) synthesis; (b) MIPs and NIPs before washing with Soxhlet system; (c) washing of MIPs with Soxhlet.
Figure 2
Figure 2
FTIR spectra of MIPs and NIPs after washed using Soxhlet system.
Figure 3
Figure 3
N2 adsorption–desorption isotherms using the BET method for MIPs and NIPs.
Figure 4
Figure 4
CV profile of 25 µmol L−1 of curcumin in a pH 2 BR buffer from modified electrodes.
Figure 5
Figure 5
The proposed mechanism for curcumin oxidation.
Figure 6
Figure 6
Differential pulse voltammograms obtained from the application of 0.4 μmol L−1 of curcumin at different pHs.
Figure 7
Figure 7
(A) Calibration curve for the detection of curcumin based on the application of MIP/MWCNT/GCE in BR buffer with pH 2. Inset: DPV analysis. (B) Calibration curve for the HPLC method.
Figure 8
Figure 8
Interference study carried out using MIP/MWCNT/GCE.
Figure 9
Figure 9
Differential pulse voltammograms obtained from the application of 0.4 μmol L−1 of curcumin; n = 10 replicates.
Figure 10
Figure 10
Quantification curve by standard addition method of real samples: capsule, plant root, and food seasoning.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Alvarado H.-L., Limón D., Calpena-Campmany A.-C., Mallandrich M., Rodríguez-Cid L., Aliaga-Alcalde N., González-Campo A., Pérez-García L. Intrinsic Permeation and Anti-Inflammatory Evaluation of Curcumin, Bisdemethoxycurcumin and Bisdemethylcurcumin by a Validated HPLC-UV Method. Int. J. Mol. Sci. 2023;24:6640. doi: 10.3390/ijms24076640. - DOI - PMC - PubMed
    1. Abdel-Kader M.S., Salkini A.A., Alam P., Alshahrani K.A., Foudah A.I., Alqarni M.H. A High-Performance Thin-Layer Chromatographic Method for the Simultaneous Determination of Curcumin I, Curcumin II and Curcumin III in Curcuma longa and Herbal Formulation. Separations. 2022;9:94. doi: 10.3390/separations9040094. - DOI
    1. Rios-Aguirre S., Gil-Garzón M.A. Microencapsulación por secado por aspersión de compuestos bioactivos en diversas matrices: Una revisión. TecnoLógicas. 2021;24:e1836. doi: 10.22430/22565337.1836. - DOI
    1. Jin T., Song Z., Weng J., Fantus I.G. Curcumin and other dietary polyphenols: Potential mechanisms of metabolic actions and therapy for diabetes and obesity. Am. J. Physiol. Metab. 2018;314:E201–E205. doi: 10.1152/ajpendo.00285.2017. - DOI - PubMed
    1. Gupta S., Babu B.V. Removal of toxic metal Cr(VI) from aqueous solutions using sawdust as adsorbent: Equilibrium, kinetics and regeneration studies. Chem. Eng. J. 2009;150:352–365. doi: 10.1016/j.cej.2009.01.013. - DOI

LinkOut - more resources