The Position of Lophozia dubia (Lophoziaceae, Marchantiophyta) in the Phylogenetic System of Lophozia and the Distribution of Lophozia in Southeast Eurasia, Extending to Indonesia

Abstract

Lophozia pallida, the commonly used name for a rare and little-known Sino-Himalayan species, was found to be a synonym of Lophozia dubia, a forgotten and previously misinterpreted species known in Indonesia. A comparative study of herbarium materials and our collections made it possible to 'extend' the distribution of Lophozia s. str. southward to Indonesia. The description of oil bodies from the species is provided for the first time. The position of the species in the Lophozia phylogenetic system demonstrates its clear differences from the morphologically similar Lophozia guttulata and its phylogenetic relationship with the Japanese-Korean Lophozia koreana.

Keywords: East Asia; ITS 1–2; Lophozia; Lophoziaceae; Southeast Asia; molecular genetics; trnL–F.

Figure 1

Phylogram obtained from a Bayesian analysis of the genus Lophozia (Dumort.) Dumort., Lophoziopsis Konstant. & Vilnet and related taxa based on trnL–F. Newly obtained specimen is marked in red. Bootstrap support values > 50% in ML analysis and Bayesian posterior probabilities PP > 0.50 are indicated. Scale bar denotes the number of nucleotide substitutions per site.

Figure 2

Phylogram obtained from a Bayesian analysis of the genus Lophozia (Dumort.) Dumort., Lophoziopsis Konstant. & Vilnet and related taxa based on ITS 1–2. Newly obtained specimen is marked in red. Bootstrap support values > 50% in ML analysis and Bayesian posterior probabilities PP > 0.50 are indicated. Scale bar denotes the number of nucleotide substitutions per site.

Figure 3

Phylogram obtained from a Bayesian analysis of the genus Lophozia (Dumort.) Dumort., Lophoziopsis Konstant. & Vilnet and related taxa based on a combined ITS–trnL–F dataset. Newly obtained specimen is marked in red. Bootstrap support values > 50% in ML analysis and Bayesian posterior probabilities PP > 0.50 are indicated. Scale bar denotes the number of nucleotide substitutions per site.

Figure 4

Lophozia dubia Schiffn. (A) upper part of a shoot fragment, side view; (B) upper part of a shoot fragment, dorsal view; (C) middle cells of leaf; (D) stem cross-section, fragment; (E–J) leaves. Scales: a—1 mm for (A,B); b—50 µm for (C); c—200 µm for (D); d—1 mm for (E–J). All from V. Schiffner, 605 (FH).

Figure 5

Lophozia dubia Shiffn. (A,C) part of mat.; (B) complex mat. (Lophozia dubia is intermixed here with Anastrepta orcadensis (Hook.) Schiffn. and Mylia vietnamica Bakalin & Vilnet); (D–F) midleaf cells with oil bodies; (G) leaf. Scales: 2 mm for (A–C); 50 µm for (D–F); 1 mm for (G). All from C-83-7-18 (VBGI).

Figure 6

Lophozia dubia Schiffn. (A,B) upper part of a shoot fragment, dorsal view; (C) stem cross-section fragment; (D–M) leaves. Scales: a—1 mm for (A,B); b—100 µm for (C); c—1 mm for (E,H–J); d—1 mm for (D,F,G,K–M). All from C-83-7-18 (VBGI).

Figure 7

Lophozia dubia Schiffn. (A) label; (B) shoot fragments, dorsal view. Scale: 2 mm for (B). All from 7131 JE04009230 (lectotype of Lophozia handelii Herzog).

Figure 8

Lophozia dubia Schiffn. (A,C) leaves; (B,D) leaf apex cells; (E) midleaf cells with oil bodies. Scales: 1 mm for (A,C); 50 µm for (B,D,E). All from 7131 JE04009230 (lectotype of Lophozia handelii Herzog).

Figure 9

Lophozia dubia Schiffn. (A,B) middle part of a shoot fragment, side view; (C) leaf apex cells; (D) leaf middle cells; (E–G) leaves. All from G00053383/10810 (holotype of Anastrophyllum pallidum Steph.). (H) upper part of a shoot fragment, dorsal view; (I–M) leaves; (N) leaf middle cells; (O–R) gemmae. All from PC102533 (isolectotype of Lophozia handelii Herzog.). Scales: (a,c)—2 mm for (A,B,H); (b,g)—1 mm for (E–G,I–M); d,e,f,h—50 µm for (C,D,N,O–R).

Figure 10

Lophozia dubia Schiffn. (A,D) leaf middle cells with oil bodies; (B,C,F) leaves; (E) leaf apex cells; (G,H) shoot fragments. Scales: 1 mm for (B,F); 2 mm for (C,G,H); 50 µm for (A,D,E). (A,B,F) from G00121692; (C–E,G,H) from Miehe 6993d.

Figure 11

Lophozia dubia Schiffn. (A,B) leaves; (C) leaf apex cells; (D) shoot fragments. Scales: 500 µm for (A,B); 50 µm for (C); 2mm for (D). (A–C) from Miehe 6993d; (D) from Li X.J.920.