Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2024 Feb 1;13(3):436.
doi: 10.3390/plants13030436.

Efficient Single Nucleotide Polymorphism Marker-Assisted Selection to Fusarium Wilt in Chickpea

Patricia Castro  1 , Cristina Caballo  2 , Alejandro Carmona  1 , Teresa Millan  1 , Juan Gil  1 , José V Die  1 , Inmaculada Izquierdo  3 , Josefa Rubio  2
Affiliations

Efficient Single Nucleotide Polymorphism Marker-Assisted Selection to Fusarium Wilt in Chickpea

Patricia Castro et al. Plants (Basel). .

Abstract

Fusarium wilt is one of the most destructive chickpea diseases worldwide. Race 5 (Foc5) is the most harmful in the Mediterranean basin. The primary objective of this study is to validate a block of six SNP markers previously mapped in Ca2 in a diverse panel of cultivars, advanced and inbred lines phenotyped for resistance to fusarium wilt. Additionally, we aim to assess the effectiveness of using these markers in the selection of resistant Foc5 lines in an ongoing breeding program. The results showed a 100% coincidence between phenotype and expected haplotype in plant material evaluated for Foc5. We also analyzed 67 inbred lines previously phenotyped by different authors for fusarium wilt reaction, though the specific race was not specified. In these accessions, 65.8% of the analyzed lines exhibited complete correspondence between the phenotype and haplotype. Our results suggest that in early generations it is possible to select resistant materials with reliability, leading to the removal of a significant number of lines, thereby reducing costs and facilitating the handling of materials for additional trait evaluations. Functional annotation of genes delimited by the SNP block revealed several genes in the "response to stimulus" category with potential roles in the resistance reaction.

Keywords: SNPs; breeding; chickpea; fusarium wilt; marker assisted selection.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflicts of interest.

Figures

Figure 1
Figure 1
Gene ontology “biological process” category distribution of annotated genes in the genomic region delimited by the SNP markers.
See this image and copyright information in PMC

References

    1. Sharma K.D., Muehlbauer F.J. Fusarium wilt of chickpea: Physiological specialization, genetics of resistance and resistance gene tagging. Euphytica. 2007;157:1–14. doi: 10.1007/s10681-007-9401-y. - DOI
    1. Li H., Rodda M., Gnanasambandam A., Aftab M., Redden R., Hobson K., Rosewarne G., Materne M., Kaur S., Slater A.T. Breeding for biotic stress resistance in chickpea: Progress and prospects. Euphytica. 2015;204:257–288. doi: 10.1007/s10681-015-1462-8. - DOI
    1. Jendoubi W., Bouhadida M., Boukteb A., Béji M., Kharrat M. Fusarium Wilt affecting chickpea crop. Agriculture. 2017;7:23. doi: 10.3390/agriculture7030023. - DOI
    1. Tekeoglu M., Tullu A., Kaiser W.J., Muehlbauer F.J. Inheritance and linkage of two genes that confer resistance to Fusarium wilt in chickpea. Crop Sci. 2000;40:1247. doi: 10.2135/cropsci2000.4051247x. - DOI
    1. Gowda S.J.M., Radhika P., Kadoo N.Y., Mhase L.B., Gupta V.S. Molecular mapping of wilt resistance genes in chickpea. Mol. Breed. 2009;24:177–183. doi: 10.1007/s11032-009-9282-y. - DOI

LinkOut - more resources