Evaluation of Black Soldier Fly Larvae Meal on Growth, Body Composition, Immune Responses, and Antioxidant Capacity of Redclaw Crayfish (Cherax quadricarinatus) Juveniles

Abstract

This study investigated the effects of substituting fish meal (FM) with black soldier fly (Hermetia illucens) meal (BSM) on the growth performance, body composition, immune response, and antioxidant enzyme activity of juvenile redclaw crayfish (Cherax quadricarinatus). Four isonitrogenous (41%) and isolipidic (11%) diets (i.e., FM substitutes) were formulated from BSM prepared using larvae that were fed soybean meal (BSM-S), fishery byproducts (BSM-F), or pitaya (BSM-P). The experimental diets were fed twice daily to triplicate groups of juvenile redclaw crayfish (0.56 ± 0.04 g). After the feed trial, the FM and BSM-F groups exhibited significantly lower feed conversion ratios and significantly higher weight gain; specific growth rates; and concentrations of saturated fatty acids, highly unsaturated fatty acids, eicosapentaenoic acid, and docosahexaenoic acid in the muscle. Among the tested groups, the BSM-F group exhibited significantly enhanced immune responses and increased antioxidant enzyme activity (i.e., superoxide dismutase, phenoloxidase, and glutathione peroxidase); the BSM-P group exhibited a significantly higher feed intake and hepatopancreatic index; and the FM group exhibited a significantly higher muscle body index and apparent digestibility for the dry matter of crude protein. The findings indicate that the juvenile redclaw crayfish fed BSM-F achieved the highest weight gain among the groups.

Keywords: Decapoda; fish meal; insect meal; organic waste.

Figure 1

Hemocyte respiratory burst of redclaw crayfish fed different experimental diets for 56 days. Data are expressed as mean ± standard error values (10 redclaw crayfish per tank; n = 3), and different letters indicate significant differences among groups (p < 0.05).

Figure 2

Hemocyte lysozyme activity of redclaw crayfish fed different experimental diets for 56 days. Data are expressed as mean ± standard error values (10 redclaw crayfish per tank; n = 3), and different letters indicate significant differences among groups (p < 0.05).