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. 2024 Jan;176(3):394-398.
doi: 10.1007/s10517-024-06031-0. Epub 2024 Feb 12.

Influence of a Passive Tilt Test on the Proteomic Composition of the Blood of Healthy Humans

D N Kashirina  1 , L Kh Pastushkova  2 , A S Kononikhin  2 , A G Brzhozovskiy  2 , I M Larina  2
Affiliations

Influence of a Passive Tilt Test on the Proteomic Composition of the Blood of Healthy Humans

D N Kashirina et al. Bull Exp Biol Med. 2024 Jan.

Abstract

In order to identify changes in the blood proteome of healthy volunteers after passive tilt test carried out on day 19 of head-down bed rest, a chromato-mass-spectrometric analysis of samples of dried blood spots was carried out. It was revealed that the body's response to the tilt test was characterized by a decrease in the level of HDL and kininogen-1. After the tilt test, we observed an increase in the level of vimentin, vitamin K-dependent protein C, Wnt signaling pathway proteins, proteins involved in autophagy and adaptive immune response, focal adhesion proteins, vascular damage marker S100A8, PEDF regulator, and some proteins of the heart: cardiac actin ACTC1 and transcription factor GATA4. The obtained results lay the foundation for future research in the framework of identifying the risks of developing cardiovascular changes in astronauts after space flights.

Keywords: dried blood spots; passive tilt test; proteome.

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