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. 2024:2763:101-109.
doi: 10.1007/978-1-0716-3670-1_8.

Supported Immunohistochemical Staining of Mucins

Michiyo Higashi  1
Affiliations

Supported Immunohistochemical Staining of Mucins

Michiyo Higashi. Methods Mol Biol. 2024.

Abstract

Immunohistochemistry (IHC) staining is the most common method to detect the distribution and localization of biomarkers in different parts of a tissue. Antibodies for tandem repeat peptide of mucins are very popular, but antibodies for glycosylation or others are also used. IHC for mucin is the same protocol as IHC for others. This description includes IHC according to ABC method for processed formalin-fixed paraffin-embedded (FFPE) tissues. Protocol of in situ hybridization is also shown.

Keywords: Avidin-Biotin conjugate; Immunohistochemistry; In situ hybridization; Indirect method; Mucins.

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