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. 2024:2763:345-352.
doi: 10.1007/978-1-0716-3670-1_29.

Measurement of Mucinase Activity

Hiroki Tanabe  1 , Tatsuya Morita  2 , Naomichi Nishimura  3
Affiliations

Measurement of Mucinase Activity

Hiroki Tanabe et al. Methods Mol Biol. 2024.

Abstract

Mucinase consists of some proteases, glycosidases, sulfatases, and sialidases. It is not practical to measure individual enzyme activities when measuring mucinase activity. In this method, mucinase activity is measured using porcine gastric mucin as a substrate and feces as an enzyme source. This description includes fecal pellet preparation, reaction procedure of mucinase, measurement of reducing sugars liberated during the procedure, and determination of nitrogen content in the fecal preparations.

Keywords: Feces; Mucinase; Porcine gastric mucin; Rat.

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